针刀疗法对缺血缺氧性脑损伤幼鼠胶质纤维酸性蛋白表达的影响

Influence of Acupotomy Therapy on Glial Fibriliary Acidic Protein Expression in Neonatal Rats with Hypoxia-Ischemic Brain Injury

【目的】观察针刀疗法对缺血缺氧性脑损伤幼鼠胶质纤维酸性蛋白(GFAP)表达的影响。【方法】选用7日龄新生SD大鼠随机分为针刀组、模型组、假手术组,针刀组于造模后2、9、16 d各治疗1次,造模后第17天通过悬吊试验观察缺血缺氧性幼鼠随意运动,并采用苏木精—伊红(HE)及免疫组织化学染色观察缺血缺氧性幼鼠脑组织的病理变化和GFAP表达。【结果】(1)悬吊试验评分结果显示:模型组评分显著降低,与假手术组比较差异有统计学意义(P<0.05),表明模型组随意运动有异常;针刀组与假手术组比较差异无统计学意义(P>0.05),表明针刀组随意运动接近正常。(2)HE染色显示:模型组病侧脑组织神经细胞水肿、变性、坏死显著,针刀组上述病理变化有所减轻。GFAP免疫组织化学染色显示:模型组出现GFAP阳性细胞增生,染色较深,染色范围较广;针刀组GFAP阳性细胞增生减轻,染色变浅,染色范围亦变小;假手术组未见GFAP阳性细胞增生,染色浅,染色范围分布稀疏。定量分析结果显示:模型组阳性细胞面密度显著升高,与假手术组比较差异有统计学意义(P<0.05);针刀组阳性细胞面密度显著降低,与模型组比较差异有统计学意义(P<0.05),表明针刀组GFAP表达较模型组显著减少。【结论】针刀疗法能够改善幼鼠缺血缺氧性脑损伤引起的随意运动障碍,减轻中枢神经细胞损伤和GFAP阳性细胞增生,下调GFAP表达。

Objective To observe the influence of acupotomy therapy on glial fibriliary acidic protein （GFAP） expression in neonatal rats with hypoxia-ischemic brain damage （HIBD）. Methods Seven-day-old SD rats were randomized into sham operation group, model group and acupotomy group. After the models were established successfully, acupotomy therapy was given to the model rats once every 7days from the second day. On day 17 after modeling, the voluntary motor function of the rats was observed by hanging over test, the pathological changes of their brain were examined after hematoxylin-eosin （HE） staining, and cerebral GFAP expression was detected by immunohistochemical staining. Results （ 1 ） There was statistical significance in hanging over test scores between the model group and sham operation group （P 〈 0.05 ）, but no statistical significance between acupotomy group and sham operation group （ P 〉 0.05 ）, indicating that voluntary motor function in acupotomy group was nearly recovered. （2） The results of HE staining showed that cerebral neurons had obvious edema, degeneration and necrosis in the model group, and then the pathological changes of neurons were relieved in acupotomy group. The number of GFAP positive cells increased dramatically and were stained thickly and extensively in the model group, and then the above changes were relieved after acupotomy treatment, the differences being statistically significant between the two groups （P 〈 0.05 ）. GFAP positive ceils showed no proliferation, and the staining was thin in the sham operation group. The results of quantitative analysis showed that the density of GFAP positive cells was higher in the model group than that in sham operation group （P 〈 0.05 ）, and then was decreased in acupotomy group （ P 〈 0.05 compared with that in the model group）, indicating that GFAP expression was decreased in acupotomy group. Conclusion Acupotomy exerts certain effect on relieving disability of voluntary motor function caused by HIBD in neonatal rats, and on reducing nerve cell injuries , GFAP-positive cell proliferation and GFAP expression.