耐辐射异球菌pprM基因克隆及功能预测

Gene Cloning of Deinococcus radiodurans pprM and Its Function Prediction

【目的】通过耐辐射异球菌(Deinococcus radiodurans)pprM基因可能功能的研究,为进一步开发利用耐辐射微生物宝贵基因资源奠定基础。【方法】通过PCR方法克隆pprM基因,利用穿梭表达载体pRADZ3将其转入大肠杆菌内,通过冲击试验分析其功能。【结果】pprM基因转入大肠杆菌,发现该基因能提高大肠杆菌的抗盐、耐旱能力。【结论】pprM基因是一个非常重要的抗逆基因,在农业育种方面有极好的应用前景。

[ Objective ] Through function studies on pprM gene of the radiation - resistance bacteria Deinococcus radiodurans, to lay the foundation for further development and utilization of radiation resistant microbe precious genetic resources. [ Method] Clone the pprM gene by PCR, then transfer it into Escherichia coli though the shuttle expression vector pRADZ3, and its function is analyzed by shock test. [ Result ] It is found that pprM gene can improve the salt resistance and drought - resistant ability of E. coli. [ Conclusion ] pprM gene is a very important stress -tolerance gene, which has an excellent application prospects in agricultural breeding.