摘要
西尼罗病毒可引起人畜共患虫媒病,已经在全球许多国家流行。主要随迁飞的鸟类携带扩散,传入我国的风险很大。本研究在参考多株西尼罗病毒基因序列的基础上,设计引物及探针,建立了实时荧光RT-PCR的方法。特异性试验表明,该方法对同为黄病毒科的日本脑炎病毒(JEV)SA14-14-2株和登革病毒(DEN)Ⅰ型cDNA检测均为阴性;敏感性试验证实,实时RT-PCR检测敏感度要比常规RT-PCR法高100倍。说明本研究建立的实时RT-PCR方法敏感性和特异性均较高,可用于西尼罗病毒感染的早期监测和诊断。
West Nile virus （WNV） is by far the most widely distributed arbovirus. The WNV infection has been recognized as an emerging infectious disease with possible transmitting risk in our country. The pairs of primers and TaqMan probes were designed and synthesized based on the nucleotide sequences of WNV in GenBank. After optimization, the real-time PCR was established. These primers were applied to detect the nucleic acids of WNV, Japanese B encephalitis virus （JEV） and Dengue viruses （DEN） , and the latter two viruses could not be detected. Compared with conventional RT-PCR, real-time PCR appeared to be approximately 100 times more sensitive in detection of WNV. It suggests that the real-time PCR developed for the detection of WNV shows high degree of specificity and sensitivity, and can be used for WNV inspection and investigation.
出处
《畜牧与兽医》
北大核心
2012年第9期4-7,共4页
Animal Husbandry & Veterinary Medicine
基金
上海市科委基金(05dz05005)
