慢性铝中毒对大鼠端脑和海马中糖原合成酶激酶3β的影响

Influence of chronic aluminum poisoning on the glycogen synthase kinase 3 beta in rat endbrain and hippocampi

目的探讨慢性铝中毒大鼠端脑和海马中糖原合成酶激酶3β(GSK-3β)的表达。方法 6月龄雌性SD大鼠60只随机分为正常组、口服铝组和腹腔注射铝组,每组20只。正常组饲喂正常饲料,口服铝组饲喂25 mg.g-1的含AlCl3.6H2O饲料,腹腔注射铝组腹腔注射9 g.L-1的灭菌AlCl3.6H2O溶液,每周3次。3个月后,股动脉放血处死大鼠。酶联免疫吸附测定法检测海马细胞中GSK-3β蛋白的表达;反转录聚合酶链反应检测海马和端脑中GSK-3β基因的表达。结果与正常组比较,GSK-3βmRNA在口服铝和腹腔注射铝组大鼠端脑和海马中均高表达(P<0.05);腹腔注射铝组海马中GSK-3βmRNA表达高于口服铝组(P<0.05)。口服铝组和腹腔注射铝组大鼠海马中GSK-3β蛋白表达与正常组比较差异无统计学意义(P>0.05)。结论慢性铝中毒后,端脑与海马中GSK-3β基因表达水平均明显升高。

Objective To investigate the expression of glycogen synthase kinase 3 beta（GSK-3β） in rat endbrain and hippocampi with chronic aluminum poisoning.Methods Sixty female SD rats,6 months old,were randomly divided into normal group,aluminum taking orally group and intraperitoneal injection aluminum group,20 rats in each group.Normal group was fed with general forage.Aluminum taking orally group was given forage containing AlCl3·6H2O 25 mg·g-1.Intraperitoneal injection aluminum group was injected sterilized 9 g·L-1 AlCl3·6H2O solution,3 times a week.Three months later,rats were sacrificed by femoral artery bleeding.The expression of GSK-3β protein in hippocampus was detected by enzyme-linked immunosorbent assay and the expression of GSK-3β gene in endbrain and hippocampi was detected by reverse transcription polymerase chain reaction.Results Compared with the normal group,GSK-3β mRNA showed higher expression in the rat endbrain and hippocampi in both aluminum taking orally group and intraperitoneal injection aluminum group（P0.05）.Conclusion GSK-3β gene expression level in rats endbrain and hippocampi obviously increase after chronic aluminum poisoning.