摘要
目的:检测HER2基因拷贝数的增加与结肠腺癌的发生是否存在关联。方法:收集了134例结肠腺癌组织,并以其配对的自身对照正常组织作为对照。运用DNA提取试剂盒提取其组织中DNA,运用PCR和实时定量PCR检测其HER2基因拷贝数及其mRNA的表达水平。结果:35.1%的结肠腺癌患者组织中HER2基因拷贝数与其正常自身对照相比,其数目明显增加,且有统计学意义,并且,其HER2基因拷贝数随着病程的进展而逐渐增高。而且,HER2基因拷贝数的增加也会上调其组织中mRNA的表达水平。方论:在结肠腺癌中,HER2基因拷贝数的增加可作为一个潜在的检测指标,为临床早期诊断和发现提供一些理论基础。
Objective: To investigate the correlation between copy-number variations(CNVs) of HER2 gene and colorectal cancer(CRC).Methods: Case-matched tissues of colorectal adenocarcinomas and adjacent normal epithelium(n=134) were included in this study.PCR and quantitative PCR was carried out to examine the copy number and mRNA expression of HER2 in CRCs.Results: Increased copy numbers of HER2 were detected in a relative high percentage of CRC samples(35.1%,47 out of 134).There was a positive correlation between copy number increase of HER2 and tumor progression,and also,increased HER2 copies showed a positive correlation to mRNA over-expression in CRCs.Conclusion: These findings suggest that copy number increase of HER2 has the potential to serve as a diagnostic indicator,alone or in combination with other markers,for CRC.
出处
《武汉大学学报(医学版)》
CAS
北大核心
2012年第5期710-713,716,共5页
Medical Journal of Wuhan University