HER2基因拷贝数增加与结肠腺癌发生的关联性

Correlation Between Increased HER2 Copy Number and Colorectal Cancer

目的:检测HER2基因拷贝数的增加与结肠腺癌的发生是否存在关联。方法:收集了134例结肠腺癌组织,并以其配对的自身对照正常组织作为对照。运用DNA提取试剂盒提取其组织中DNA,运用PCR和实时定量PCR检测其HER2基因拷贝数及其mRNA的表达水平。结果:35.1%的结肠腺癌患者组织中HER2基因拷贝数与其正常自身对照相比,其数目明显增加,且有统计学意义,并且,其HER2基因拷贝数随着病程的进展而逐渐增高。而且,HER2基因拷贝数的增加也会上调其组织中mRNA的表达水平。方论:在结肠腺癌中,HER2基因拷贝数的增加可作为一个潜在的检测指标,为临床早期诊断和发现提供一些理论基础。

Objective： To investigate the correlation between copy-number variations（CNVs） of HER2 gene and colorectal cancer（CRC）.Methods： Case-matched tissues of colorectal adenocarcinomas and adjacent normal epithelium（n=134） were included in this study.PCR and quantitative PCR was carried out to examine the copy number and mRNA expression of HER2 in CRCs.Results： Increased copy numbers of HER2 were detected in a relative high percentage of CRC samples（35.1%,47 out of 134）.There was a positive correlation between copy number increase of HER2 and tumor progression,and also,increased HER2 copies showed a positive correlation to mRNA over-expression in CRCs.Conclusion： These findings suggest that copy number increase of HER2 has the potential to serve as a diagnostic indicator,alone or in combination with other markers,for CRC.