基质金属蛋白酶-12血清水平及启动子区-82A／G多态性与急性动脉粥样硬化性脑梗死的关系

Association of serum levels of matrix metalloproteinase-12 and a functional polymorphism in the promoter -82A/G of the matrix metalloproteinase-12 gene with acute atherosclerotic cerebral infarction

目的探讨基质金属蛋白酶-12（MMP-12）血清水平及启动子基因-82A／G多态性与动脉粥样硬化性脑梗死的关系。方法对608例动脉粥样硬化脑梗死和374名健康体检者进行研究，采用ELISA法测定血清MMP-12蛋白水平，同时采用聚合酶链反应-限制性片段长度多态性（PCR—RFLP）方法分析MMP-12基因启动子区-82A／G多态性。结果脑梗死组患者48h内血清MMP-12为（17．36±9．12）ng／ml，对照组为（17．42±7．70）ng／ml，两组比较差异无统计学意义（t=0．047，P=0．962）。脑梗死组AG＋GG基因型频率为7．6％，与对照组的5．9％基因型频率比较差异无统计学意义（χ^2=0．281，P=0．584），G等位基因频率在两组间分别为3．8％和2．9％，两组比较差异同样无统计学意义（χ^2=0．746，P=0．374）。结论MMP-12血清水平及基因启动子-82位点的多态与MMP-12基因表达和脑梗死没有相关性。

Objective To investigate the association of serum levels of matrix metalloproteinase-12 （MMP-12） and a functional polymorphism in the promoter -82A/G of the MMP-12 gene with acute atherosclerotic cerebral infarction （ACI） . Methods All 608 cases of acute ACI and 374 healthy controls were included in the study. Serum levels of MMP-12 were measured using the enzyme-linked immunosorbent assay （ELISA）. At the same time, polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was performed on the - 82A/G polymorphism in the MMP-12 gene. Results The serum levels of MMP-12 in ACI group （（ 17.36±9. 12） ng/ml） were not significantly higher than those in the healthy control group （ （ 17.42±7. 70） ng/ml, t = 0. 047, P = 0. 962）. The frequency of the AG ＋ GG genotypes was not significantly different between the two groups （7.6% vs 5.9 % , χ^2 = 0. 281, P = 0. 584 ） , and the frequencies of the G allele were 3.8% and 2. 9% in the ACI group and the control group respectively which were also not significantly different between the two groups （ χ^2 = 0. 746, P = 0. 374 ）. Conclusion There is no correlation between serum level with genetic polymorphism in MMP-12 promoter （ - 82A/G） and cerebral infarction.