高分辨魔角旋转核磁共振技术在肿瘤代谢组研究中的应用

Applications of high resolution magic angle spinning NMR spectroscopy in tumor metabonomics research

高分辨魔角旋转核磁共振技术(HRMAS)无需溶剂提取分离,能直接检测离体生物组织样品,得到较高分辨率的核磁共振图谱,在代谢组研究中得到广泛应用。应用HRMAS检测脑、肝、肾、肺等多种离体肿瘤组织,可以直接得到组织细胞中分子水平的代谢物结构和组成信息。基于这些优势,HRMAS在肿瘤早期诊断研究方面展现出良好的应用前景。本文主要介绍了HRMAS技术在肿瘤组织代谢组研究中的应用。

The high resolution magic angle spinning NMR spectroscopy （HRMAS） can be directly used to examine in vitro tissues, needing no extracting and separating procedures. Moreover, it can enhance signal intensity and improve resolution of images. HRMAS can directly obtain the structural and conformational information （molecular level） of in vitro tumor samples of the brain, liver, kidney, and lung, which gives it a bright future in early diagnosis of tumors. The present paper mainly reviews the applications of HRMAS in tumor metabonomics research