大黄对伴高脂血症急性胰腺炎大鼠肠道细菌移位干预作用的研究

Effect of Rhubarb on intestinal bacterial translocation in rats with hyperlipoidemia and acute pancreatitis

目的:观察大黄对伴高脂血症急性胰腺炎(HLAP)大鼠肠道细菌移位的干预作用。方法:60只SD大鼠随机分为5组:对照组、AP组、HLAP组、AP治疗组和HLAP治疗组。建立大鼠AP及HLAP模型,治疗组予大黄治疗,检测大鼠血清淀粉酶及TG、肠道通透性、小肠形态学改变;取标本(门静脉血、胰腺及肠系膜淋巴结)进行细菌分析。结果:术后24h HLAP组及HLAP治疗组淀粉酶低于AP组及AP治疗组(P<0.05),术后6d治疗两组淀粉酶均低于术后24h(P<0.01);术后24h HLAP组及HLAP治疗组TG高于余3组(P<0.01),术后6d各组均有下降,HLAP治疗组最明显。术后第3天HLAP组L/M高于AP组(P<0.05),AP治疗组低于AP组(P<0.05),HLAP治疗组明显低于HLAP组(P<0.01)。AP组、HLAP组小肠绒毛肿胀破坏,固有层炎性细胞浸润,两治疗组肠黏膜损害较轻。与AP组比较,HLAP组组织培养及门静脉血16srRNA阳性率均明显升高(P<0.01),AP治疗组、HLAP治疗组细菌阳性率分别明显低于AP组、HLAP组(P<0.01)。结论:HLAP肠道细菌移位较单纯AP严重,大黄能降低血脂,对HLAP肠道细菌移位有良好的疗效。

AIM： To observe the effect of rhubarb on intestinal bacterial translocation in rats with hyperlipoidemia and acute pancreatitis. METHODS： 60 SD rats were randomly divided into 5 groups： control group, AP group, HLAP group, AP treatment group, HLAP treatment group. We established rats model of AP and HLAP, rats in treatment groups were treated by rhubarb. The levels of serum amylase and tri-glyeeride, intesital permeability, mucosa struc- ture of small intestine were checked. Portal vein blood, pancreas and mesenteric lymph node were collected for 16s rRNA gene and culture. RE-SULTS： The levels of serum amylase in HLAP group and HLAP treatment group were lower than in AP group and AP reatment group 24h af-ter the operation （P〈0.05）. The serum amyl-ase 6 days after the operation in both treatment groups were lower than those 24 h after the op-eration （P〈0.01）. The levels of serum triglyc-eride were significant high in HLAP group and HLAP treatment group compared to the other 3 groups 24 h after the operation （P〈0.01）, the levels in all 5 groups fell 6 days after the opera-tion and it was most obvious in HLAP treatment group. L/M was higher in HLAP group than in AP group 3d after the operation （P〈0.05）. L/M in AP treatment group was lower than in AP group （P〈0.05）, and L/M in HLAP treatment group was lower than in HLAP group （P〈0.01）. In AP group and HLAP group, villi of small intestine was engorged and destroyed, proper layer was infiltrated by inflammatory cells , mucosa damage was mild in both treat-ment groups. Positive rate of culture and 16s rRNA gene in HLAP group were significantly higher than AP group （P〈0. 01）. Positive rate in AP treatment group and HLAP treatment group were lower than AP group and HLAP group respectively （P 〈 0.01 ）. CONCLUSION： Compared to AP ,the intestinal bacterial trans- location in HLAP is severe. Rhubarb can treat hyperlipoidemia in HLAP, and it has obvious effect on intestinal bacterial transloeation.