多元回归模型对泽泻调脂颗粒组方的定量评价

The quantitative evaluation of the Zexie Tiaozhi granule formulation through multiple regression model

目的:基于泽泻调脂颗粒不同配伍对高脂血症大鼠模型影响的实验数据,采用非线性混合效应模型方法进行再分析,定量评价其配伍规律,为优化组方提供参考。方法:泽泻调脂颗粒4个主要成分通过均匀设计,药效指标为大鼠血清中甘油三酯(TG),采用非线性混合效应模型(NONMEM)分析。结果:最终模型提示X1为主要起效成分,X3为拮抗组分,X2与X4单独量效关系不明确,疗效主要体现于交互作用上。综合以上3点,当X1、X2、X4取最大剂量,X3取最小剂量时,组方有最大药效。结论:采用NON-MEM分析方法,可全面考虑各组分间交互作用及个体间和个体内误差等随机效应,提供的信息量更大。

AIM： The nonlinear mixed effect model （NONMEM） was applied in the analysis of Traditional Chinese Medicine （TCM） formu-la, based on the data of different compatibility of Zexie Tiaozhi granule in treatment of hyperlipi-demia rat model. The compatible regularity of Zexie Tiaozhi granule was evaluated quantita-tively and the result can be used as a reference to formula optimization. METHODS.. The four main compositions of Zexie Tiaozhi granule were combined in uniform design and analyzed by NONMEM. The TG in serum was treated as the pharmacodynamics index. RESULTS. Final model suggested that X1 was the primary effective com-position and X3 was an antagonistic composition. The dose-effect relationship of X2 and X4 was not clear, and the efficacy was mainly expressed in the interaction between compositions. Based on the above, we recommended that the formula will perform maximum efficacy when X1 , X2 and X4 take the maximum dose and X3 take the mini-mum dose. CONCLUSION. The application of NONMEM in analysis of TCM formula can in vestigate the interactions among compositions and the random effects like inter individual and intra individual variations comprehensively, which can provide vast quantities of information.