摘要
[目的 ] 了解我省腹泻患者中是否存在EAggEC感染。 [方法 ] 采用放射性α3 2 P标记的EAggEC核酸探针与分离菌杂交 ,并作HeLa细胞粘附试验。 [结果 ] 从某婴儿腹泻患者粪便分离出EAggEC ,该菌培养特征及生化反应与普通大肠杆菌基本相同 ,IMVC ++--;核酸探针及细胞粘附试验均为阳性 ,该菌对昆明小鼠表现较强的致病力。 [结论 ] 首次在我省分离到EAggEC。
Objective] In order to understand if there was EAggEC infection in the diarrheal patients in Hulan Province. [Methods] The test of the isolated bacteria hybridzed with the EAggEC nucleusic acid probe marked with radioactivity α 32 P and the test of Hela Cell adherence were Performed. [Result] A strain EAggEC was isolated from the stool samples of a boby diarrhead patient. This bacteria's training featune and biachemical rection were like the ordinary E. coli. IMVC ++--; The test of nucleus acid probe and the cell adherence was positive. This bacteria was strongly Pathogentic to Kunming mice. [Conclusion] EAggEC was first isolated in Hulam Province.
出处
《实用预防医学》
CAS
2000年第2期93-95,共3页
Practical Preventive Medicine
基金
湖南省卫生厅科研资助!项目[湘卫科教发 (1 996) 7号 ]