辽宁发现库蚊黄病毒

Isolation of the Culex Flavivirus from Mosquitoes in Liaoning Province,China

2011年从辽宁省丹东地区蚊虫样品中分离到6株病毒,采用逆转录-聚合酶链式扩增(RT-PCR)检测方法,结果黄病毒属通用引物和库蚊黄病毒特异性引物均为阳性。6株病毒核苷酸序列经基因库(GenBank)比对后,证实6株病毒为库蚊黄病毒,为我国首次报道。将病毒NS5基因和E基因核苷酸序列与GenBank中10株库蚊黄病毒参考毒株核苷酸序列进行比较,并构建遗传进化树,结果显示本次分离的6株病毒与美国和日本的毒株亲缘关系较近。

. A flavivirus, Culex flavivirus, was first isolated from Chinese mosquitoes with high sequences similarities to those of flaviviruses found in America and Japan. In this study, a total of 48 pools of field- collected mosquitoes were sampled from Dandong of Liaoning Province, China during July to September of 2011. Six isolated viruses showing cytopathic effect（CPE）in C6/C36 cells were tested by reverse transcrip- tion polymerase chain reaction（RT-PCR）using Flavivirus genus - specific primers and Culex flavivirusspecific primers and the positive PCR-product was sequenced and compared with the sequences of 10 iso lates from GenBank. Phylogenetic tree of NS5 and enevelop genes of flavivirus were constructed. The Gen- Bank accession numbers of NS5 gene were JQ409188,JQ409186,JQ409187,JQ409191,JQ409189 and JQ409190. The GenBank accession numbers of envelope gene were JQ065883, JQ065882, JQ065881, JQ065879,JQ065877 and JQ065878.