野马追提取液对动脉粥样硬化家兔炎症反应的防治作用

Lindley eupatorium herb extract prevents and attenuates vascular inflammation reaction in atherosclerosis rabbits

目的观察野马追提取液(lindley eupatorium herb,LEH)对动脉粥样硬化(atherosclerosis,AS)家兔模型血管壁炎症的影响。方法将36只家兔完全随机分为6组:正常对照组,模型组,阳性药物组及野马追大、中、小剂量组,分别给予基础饲料,高脂饲料及高脂饲料分别添加辛伐他汀2.2 mg/kg,野马追8.34、4.17、2.08 g/kg处理。分别于6、10周检测血浆中脂质及炎性标志物C反应蛋白(C-reactive protein,CPR)、血管内皮舒张因子一氧化氮(NO)的水平,病变组织中血管细胞黏附分子-l(vascular cell adhesion molecule-1,VCAM-1)mRNA的表达;测量粥样斑块的面积及厚度。结果模型组血脂水平及主动脉粥样硬化斑块面积与正常组比较有明显升高[第10周TC:(1.39±0.23)mmol/L vs(46.30±6.73)mmol/L,P<0.05;脂质斑块面积占主动脉面积比:0%vs(89.61±7.85)%,P<0.05];野马追各组血浆中CPR及组织中VCAM-1 mRNA的表达均明显降低[(5.15±1.42)、(5.69±1.45)、(6.36±1.43)mg/L vs(8.34±1.85)mg/L,P<0.05],NO含量明显升高[(117.41±9.61)、(87.87±13.47)、(64.34±11.18)μmol/L vs(47.29±8.29)μmol/L,P<0.05]。结论野马追对动脉粥样硬化有良好的防治作用,其机制可能与抗炎相关。

Objective To determine the effects of lindley eupatorium herb （LEH） extract on the vas- cular inflammation in rabbit model with atherosclerosis. Methods A total of 36 rabbits were randomly divided into 6 groups, normal control group （treated with normal diet）, model group, positive control group and LEH groups. Rabbit model with atherosclerosis were established by high fat diet. The animals from the positive con- trol group was treated with 2.2 mg/kg simvastatin which was mixed in the diet, and those of LEH groups were treated with LEH at 8.34, 4.17 and 2.08 g/kg respectively. The serum levels of blood fat, C-reactive protein （CRP） and NO were detected in 6 and 10 weeks. The plaque area and thickness of aorta were measured with morphometry analysis when rabbits were killed after 10 weeks treatment. The damaged segments of abdominal artery were harvested for mRNA expression of vascular cell adhesion molecule-1 （VCAM-1） by RT-PCR. Results Compared with normal group, the serum level of blood fat and plague area in the model groups were significantly increased （ at the 10th week, TC ： 1.39 ± 0.23 vs 46.30 ± 6.73 mmol/L, P 〈 0. 05 ; the ratio of lipid plaque area：0% vs 89.61±7.85 %, P 〈0. 05） ; the levels of CRP in the serum and mRNA expression of VCAM-1 in the tissue of medication administration teams were significantly decreased （5.15 ± 1.42, 5.69 ± 1.45, 6.36 ± 1.43 vs 8.34 ± 1.85 mg/L, P 〈0.05）. NO content were significantly increased （117.41 ± 9.61, 87.87 ± 13.47, 64.34 ± 11.18 vs 47.29 ± 8.29 μmol/L, P 〈 0. 05）. Conclusion LEH exerts pro- tective and therapeutic effect on atherosclerosis by decreasing the level of specific markers of inflammation.