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BMP-4基因影响乳腺癌细胞增殖、凋亡和迁移能力涉及NF-κB途径 被引量：1

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摘要 目的探讨骨形态发生蛋白-4(bone morphogenetic protein 4,BMP-4)基因对人乳腺癌MCF-7细胞增殖、凋亡和迁移能力的影响途径。方法通过向乳腺癌MCF-7细胞株转染PET-BMP-4质粒过表达BMP-4;采用RT-PCR、MTT法、Boyden小室培养、Hoechst染色、Western blot方法,检测质粒转染、NF-κB抑制剂吡咯二硫代氨基甲酸盐(pyrrodlidine dthiocarbamate,PDTC)预处理和对照组细胞增殖、凋亡、迁移能力及NF-κB、Bax、Bcl-2水平。结果与空质粒转染组和空白对照组细胞相比,过表达BMP-4基因可导致MCF-7细胞增殖能力升高,凋亡细胞比例降低,细胞迁移能力增强,NF-κB和Bcl-2表达水平升高,Bax表达水平降低(P<0.05);PDTC预处理后NF-κB和Bcl-2表达水平降低,Bax表达水平升高,显著降低过表达BMP-4对MCF-7细胞的生物学效应(P<0.05)。结论过表达BMP-4基因可增强乳腺癌MCF-7细胞的增殖和迁移能力并抑制其凋亡,可能与上调NF-κB有关。
作者 朱宁生 张婧 曾晓华 江歌丽 刘晓渝
机构地区 重庆市肿瘤研究所乳腺中心 南方医科大学第二临床医学院
出处 《第三军医大学学报》 CAS CSCD 北大核心 2012年第18期1910-1912,共3页 Journal of Third Military Medical University
关键词 BMP-4 乳腺癌 增殖 凋亡 迁移 NF-ΚB
分类号 R394.2 [医药卫生—医学遗传学] R730.23 [医药卫生—肿瘤]
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参考文献11

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二级参考文献62

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第三军医大学学报
2012年 第18期

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