竹叶挥发油中主要化合物的抑菌作用

Antibacterial Effects of Major Compounds in Essential Oil from Bamboo Leaves

选取毛竹叶挥发油中含量相对较高的化合物,即正二十三碳烷、雪松脑和十六烷酸3种单体化合物,采用双层平板打孔法,研究不同质量浓度的3种单体及各单体的混合物溶液对革兰氏阳性菌(枯草芽孢杆菌)、革兰氏阴性菌(大肠杆菌、假单胞杆菌、黄杆菌)及酵母菌等菌株的抑菌功效。结果表明:雪松脑具有较强的抑菌功效,十六烷酸次之;3种单体的混合溶液亦具有较好的抑菌功效,且普遍高于单体单独作用的效果:正二十三碳烷和雪松脑混合后抑菌功效明显高于正二十三碳烷单独作用的效果,略低于雪松脑溶液的单独作用;正二十三碳烷和十六烷酸的混合液抑菌功效比正二十三碳烷溶液单独作用效果好,略低于十六烷酸溶液的单独作用;雪松脑和十六烷酸的混合液比十六烷酸溶液单独作用的抑菌功效高,略低于雪松脑溶液的单独作用;3种单体溶液混合后抑菌功效不仅比正二十三碳烷溶液和十六烷酸溶液单独作用的高,而且也比单体两两混合液的抑菌效果明显。

This paper reports the antibacterial effect of three major monomeric compounds （tricosane, cedrol and hexadecanoic acid） in the essential oil from bamboo leaves. Using agar diffusion method, the antibacterial action of these monomers and their mixtures were studied against different microorganisms： Gram-positive bacteria （Bacillus subtil＆）, Gram-negative bacteria （Escherichia coli, Pseudomonasfluorescens, Flavobacterium columnare） and yeast. The results showed that cedrol had the strongest antibacterial effect, followed by hexadecanoic acid. The antibacterial effect of combinations of the three monomers was stronger than that seen when they were separately tested. The antibacterial effect of tricosane when used in combination with cedrol was much better than that observed when it was used alone, but slightly lower than that of cedrol alone. Similarly, the antibacterial effect of tricosane was increased by combination with hexadecanoic acid, but was weaker than that of hexadecanoic acid. The antibacterial effect of a mixture of cedrol and hexadecanoic acid was higher than that observed when hexadecanoic acid was tested alone, but slightly lower than that of sole hexadecanoic acid treatment. The antioxidant effect of a mixture of the three monomers was higher than that seen when tricosane and hexadecanoic acid were tested alone, and also higher than that of combinations of two of them.