条斑紫菜藻蓝蛋白的分离纯化及其抗衰老作用研究

Separation, Purification and Anti-Aging Activity of Phycocyanin from Porphyra yezoensis

目的:分离纯化条斑紫菜藻蓝蛋白并对其进行抗衰老作用的研究。方法:以盐析沉淀法和Sephadex G-200凝胶过滤层析纯化条斑紫菜藻蓝蛋白,并对其热稳定性和酸碱稳定性进行研究。采用D-半乳糖致亚急性小鼠衰老模型,研究藻蓝蛋白的抗衰老作用。结果:在常温下,40%饱和度的硫酸铵盐析条件下,紫菜藻蓝蛋白纯度达到最大值1.73;紫菜藻蓝蛋白在30℃时、中性溶液环境时稳定性良好;藻蓝蛋白处理后均能显著提高衰老模型小鼠的胸腺指数、脾脏指数和血清中的SOD活性(P<0.05,P<0.01),显著降低血清中的MDA含量(P<0.05,P<0.0 1)。结论:紫菜藻蓝蛋白具有较强的抗衰老作用。

Objective： To isolate and purify phycocyanin from Porphyra yezoensis and explore the anti-aging activity of phycocyanin. Methods： Phycocyanin from Porphyra yezoensis was purified by salting-out and Sephadex G200 chromatography. The stability of phycocyanin under various pH and temperature conditions was also investigated. D-Galactose induced mouse models of subacute ageing were established and used to study the anti-ageing function of phycocyanin from Porphyra yezoensis. Results： Phycocyanin with the highest purity （1.73） was obtained by salting out with 40% （NH4）2SO4 at room temperature. The hycocyanin extract was stable at 30 ℃ and pH 7.0. Its oral administration resulted in a significant increased in SOD activity, thymus index and spleen index in aging mouse models （P〈0.05, P〈0.01）, and a significant reduction in serum MDA level （P 〈 0.05, P 〈 0.01）. Conclusion： Phycocyanin from Porphyra yezoensis has excellent anti-aging activity.