摘要
以植物乳杆菌(Lactobacillus plantarum)ST-Ⅲ4种胆盐水解酶(BSHs)的编码序列(bsh 1～4),将其克隆至表达载体pET-28b(+)上,在原核系统进行表达,并对其酶活力进行测定,结果发现4种BSHs的酶活力分别为29.00、20.49、24.90、21.13U/mL。同时BSH1比其他3种BSHs表现出更高的水解能力。
In vertebrates, bile salt hydrolysis plays an essential role in fat metabolism. Bile salts are synthesized in the liver. In the small intestine, glycine or taurine are de-conjugated from bile salts by the enzyme bile salt hydrolase （BSH, EC 3.5.1.24） from intestinal microbes, which reduces the serum cholesterol level. In this study, four predicted bile salt hydrolase （bsh） genes from Lactobacillus plantarum ST-III were cloned into pET-28b（＋） vector and expressed in Escherichia coli. The hydrolysis activity of these enzymes was 29.00, 20.49, 24.90 U/mL and 21.13 U/mL, respectively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第17期165-168,共4页
Food Science
基金
国家"973"计划项目(2010CB735705)
上海市科学技术委员会科研计划项目(09DZ2251400)
关键词
植物乳杆菌
胆盐水解酶
表达
酶活力
Lactobacillus plantarum
bile salt hydrolase
expression
enzyme activity
