鲍鱼菇发酵培养基的优化

Optimization of Liquid Medium for Pleurotus abalonus

采用液体培养的方法,从6种碳源和7种氮源中筛选出鲍鱼菇菌丝体生长较适的3种碳、氮源。然后选取常见的3种无机盐,运用正交试验的方法,设计三因素三水平的正交试验,筛选出鲍鱼菇菌丝体生长和胞外多糖产生的最优液体培养基。结果表明:利于鲍鱼菇菌丝体生长的液体培养基(g/100mL)为:红糖2、酵母浸粉0.3、MgSO40.1;利于鲍鱼菇胞外多糖产生的液体培养基(g/1 0 0 m L)为:果糖2、牛肉膏0.3、KH2PO4 0.1。

In this study, the appropriate carbon and nitrogen sources were selected from six carbon sources and seven nitrogen sources, respectively for optimizing liquid culture medium for the growth of mycelia of Pleurotus abalones and the production of exopolysaccharide. The optimal fermentation medium formula was evaluated by orthogonal array design method in terms of mycelium biomass and exopolysaccharide yield. The results showed that the optimal liquid medium （100 mL） for the growth of mycelia consisted of 2 g of brown sugar, 0.3 g of yeast extract and 0.1 g of MgSO4. The optimal liquid medium （100 mL） for the production of exopolysaccharide was composed of 2 g of fructose, 0.3 g of beef extract and 0.1 g of KH2PO4.