酶膜耦合连续法制备玉米肽解酒及防醉研究

Anti-Alcohol Function of Corn Peptides Produced by Continuous Enzymatic Membrane Reaction

为了考察酶膜耦合连续法制备的玉米肽(CP)在动物体内的解酒及防醉效果,并与间歇法制备的CP进行比较,采用小鼠模型分别进行翻正反射实验、解酒和防醉实验,并以市售海王金樽为阳性对照,观察小鼠的醉酒只数和醉酒时间,测定小鼠血清中乙醇体积分数及肝脏乙醇脱氢酶(ADH)的活力。结果表明:酶膜耦合连续法制备的CP能极显著地缩短小鼠醉酒时间及降低酒后血清中乙醇体积分数(P<0.01),极显著激活其肝脏ADH活力(P<0.01);各项数据均显示CP具有良好的解酒、防醉效果,连续法制备的CP其醒酒效果优于间歇法制备的CP;CP的摄入时间与饮酒时间间隔短是有利的;摄入300mg/kg(以体质量计)连续法制备的CP其解酒效果与摄入450mg/kg海王金樽相当。酶膜耦合连续法制备的CP是良好的醒酒物。

The purpose of this study was to comparatively assess the anti-alcohol function of corn peptides （CPs） produced by continuous or intermittent enzymatic membrane reaction （EMR） in animals. Righting reflex and anti-alcohol tests were carried out with mouse models and Haiwangjinzun （HWJZ）, a commercial anti-alcohol drug in China, was used as a positive control to observe ebriety rate and ebriety time. Besides, serum alcohol level and hepatic alcohol dehydrogenase （ADH） activity were measured. The results showed that CPs produced by continuous EMR could highly significantly shorten the ebriety time of mice, decrease the level of alcohol in the serum, and activate the activity of ADH in the liver （P 〈0.01）. Taken together, our results demonstrate that CPs has an excellent anti-alcohol function. Furthermore, the anti-alcohol function of CPs produced by continuous EMR is superior to that of those produced by intermittent EMR. CPs administration shortly after alcohol consumption is more effective. The anti-alcohol function of CPs produced by continuous EMR at a dose of 300 mg/kg is comparable to that of HWJZ at 450 mg/kg. Thus, CPs produce^t from continuous EMR can be considered as an excellent anti-alcohol drug.