摘要
比较研究高效毛细管电泳内标法与外标法进行含量测定结果的差异。采用高效毛细管电泳内标法(以二苯胍为内标物)和外标法分别测定三黄泻心汤3种不同剂型中盐酸小檗碱的含量,并进行了比较。外标法测得结果较内标法偏低,高效毛细管电泳内标法比外标法含量测定结果理想。
The differences in contents determination results between high performance capillary electrophoresis(HPCE) internal standard method and external standard method were compared and investigated.The contents of berberine hydrochloride in 3 kind of different preparation formulation Sanhuangxiexin decoctions by HPCE with internal standard method(with melaniline as internal standard substance) and external standard method were compared.The determined results of the external standard method were lower than that of internal standard method.The results of HPCE with internal standard method were better than external standard method.
出处
《光谱实验室》
CAS
CSCD
2012年第5期2616-2621,共6页
Chinese Journal of Spectroscopy Laboratory
基金
国家自然科学基金(30860364)
贵阳中医学院研究生教育创新计划项目(ZYYCX10029)
关键词
高效毛细管电泳
内标法
外标法
盐酸小檗碱
HPCE
Internal Standard Method
External Standard Method
Berberine Hydrochloride