HPLC测定黄芪药材中的毛蕊异黄酮葡萄糖苷

Determination of Calycosin-7-Glucoside in Astragalus membranaceus(Fisch.) Bunge by HPLC

采用高效液相色谱法(HPLC)对我国7个省68个黄芪样品进行了毛蕊异黄酮葡萄糖苷成分的含量测定。以毛蕊异黄酮葡萄糖苷含量作为衡量优劣指标,建立了黄芪药材质量标准,研究不同产地黄芪药材的质量。7个省68个黄芪药材样品检测结果表明,不同产地黄芪药材毛蕊异黄酮葡萄糖苷成分的含量差异较大,其中:山西>内蒙>甘肃>辽宁;不同品种黄芪药材毛蕊异黄酮葡萄糖苷成分的含量差异较大,其中:栽培品种<野生品种;生长年限不同,毛蕊异黄酮葡萄糖苷成分的含量也不同,其中:二年生>五年生>三年生>四年生。采用HPLC进行黄芪药材的质量评价切实可行,为制药企业选用黄芪药材提供科学、可靠的依据。

The contents of calycosin-7-glucoside of Astragalus mernbranaceus （Fisch.） Bunge samples from different producing areas were determined by HPLC with calyeosin-7-glueoside content as measurement quality index, and the medicinal materials quality standards of Astragalus membranaceus （Fisch.） Bunge was established,then the quality of Astragalus rnembranaceus（Fisch. ） Bunge from the different producing areas was studied. The 68 samples were from seven provinces in China. The detection results of 68 Astragalus mernbranaceus（Fiseh. ） Bunge from 7 provinces showed the differences of calycosin-7-glucoside componential contents were large in Astragalus rnernbranaceus （Fisch.） Bunge from different producing areas, and contents ordered as follows. Shanxi〉 Inner Mongolia〉Gansu〉Liaoning. The differences of calycosin-7-glucoside componential contents were big among different varieties of Astragalus mernbranaceus（Fiseh. ） Bunge, but the contents of eultivar varieties were less than wild varieties. The contents of ealyeosin-7-glueoside componential were different due to different growing time,and contents of growing time law the order as follows： two years 〉 five years 〉 three years 〉 four years. The quality evaluation of Astragalus rnembranaceus （Fisch.） Bunge by HPLC was available,and can provide the scientific and confident judgment for pharmacy factory in the selection of Astragalus membranaceus（Fiseh. ） Bunge.