GC法同时测定肉豆蔻中丁香酚、异丁香酚、肉豆蔻醚和榄香脂素的含量

Simultaneous determination of eugenol,isoeugenol,myristicin and elemicin in Myristica fragrans Houtt. by GC

目的:建立同时测定肉豆蔻中丁香酚、异丁香酚、肉豆蔻醚和榄香脂素4个成分含量的气相色谱法。方法:样品用乙酸乙酯超声提取,采用GC法对肉豆蔻中丁香酚、异丁香酚、肉豆蔻醚和榄香脂素进行含量测定。气相色谱条件:HP-5 MS石英毛细管柱(0.25 mm×30 m×0.25μm);氢火焰离子化检测器(FID);进样口温度240℃;检测器温度250℃;柱温起始温度为40℃,保持1 min,以5℃.min-1的速率升高到220℃,保持1 min;载气为氮气,流速1 mL.min-1,分流比为10∶1;进样量1μL。结果:丁香酚、异丁香酚、肉豆蔻醚和榄香脂素4个成分在相应的浓度范围内呈现良好的线性关系;加样回收率(n=9)分别为99.4%,99.3%,98.2%,98.8%;肉豆蔻不同部位中丁香酚、异丁香酚、肉豆蔻醚和榄香脂素的含量有明显差异。结论:本法简便快速,可以用于肉豆蔻中丁香酚、异丁香酚、肉豆蔻醚和榄香脂素4个成分的含量测定。

Objective ： To establish a GC method for simuhaneous determination of four components （eugenol, iso- eugenol, myristicin, elemicin） in Myristica fragrans. Methods： Samples were extracted with ethyl acetate using supersonic fluid extraction. GC method was applied for the determination of eugenol, isoeugenol, myristicin and el- emicin in Myristica fragrans. Chromatographic conditions ： HP - 5MS capillary column （0.25 mm x 30 m ~ 0.25 fxm） was adopted;the detector was FID;the inlet temperature was 240 ^（2 ;the detector temperature was 250 ^C ;the column temperature was programmed to start at 40 ^C, rising to 220 ^C at a rate of 5 ^C ^ rain 1 Nitrogen（ 1 mL ^ min-1） was used as the carrying gas, and the split ratio was 10：1 ;the injection volume was 1 ~L. Results： Four components（ eugenol, isoeugenol, myristicin, elemicin） had good linear relationship in the corresponding concen- tration ranges. The average recoveries（ n = 9） of the above four components were 99.4% , 99.3% , 98.2% , and 98.8%, respectively. The contents of four components in different parts of Myristicafragrans were different. Con- clusion ： This method is simple, quick and specific, which can be applied for the simultaneous determination of eu- genol, isoeugenol, myristicin, elemicin in Myristicafragrans.