鹿草根中β-蜕皮甾酮的含量测定

Determination of β-ecdysone in root of Stemmacantha carthamoides(Willd.)Dittrich

目的:建立哈萨克药鹿草根中β-蜕皮甾酮的含量测定方法。方法:采用反相,使用Waters XTerra RP C18(250 mm×4.6 mm,5μm)色谱柱,以甲醇-1%醋酸溶液(27∶73)为流动相,体积流量为1.0 mL.min-1,检测波长为250 nm,柱温30℃。结果:在上述条件下,β-蜕皮甾酮浓度在1.45～14.5μg.mL-1范围内呈良好的线性关系(r=0.9998);平均加样回收率(n=9)为100.4%。结论:本方法准确,灵敏度高,重现性好,能有效测定鹿草根中β-蜕皮甾酮含量。

Objective： To establish a method for determination of fi - ecdysone in root of Stemmacantha carthamoides（Willd. ）Dittrich. Methods：β- Ecdysone was determined by RP- HPLC on Waters XTerra RP Cls （250 mm ×4. 6mm,5 μm） column with methanol- 1% acetic acid（27：73 ）as mobile phase at a flow rate of 1.0 mL min-1, the detection wavelength was 250 nm and the column temperature was 30 ℃. Results：The calibration curve ofβ-- ecdysone was linear in the range of 1.45 -14.5 p.g mL-1 （r=0. 9998） , and the average recovery was 100.4%. Conclusions： This method is simple, accurate, sensitive and reproducible. It can be used for determi- nation ofβ-ecdysone in root of Stemmacantha carthamoides （Willd.）Dittrich.