蛹虫草菌液体培养基配方优化研究

Optimization of Cordyceps sinensis Liquid Medium

利用L9(34)正交试验设计,研究了蛹虫草菌液体培养基不同营养成分对蛹虫草菌丝生长干质量的影响,以对蛹虫草液体培养基配方进行优化。结果表明,蛹虫草液体培养基最佳配方为:玉米粉1%、麦麸1%、葡萄糖2%、蛋白胨2%、酵母膏0.5%、磷酸二氢钾0.1%、硫酸镁0.05%、维生素B110mg·L-1。蛹虫草菌最终菌体总干质量达到14.85g·L-1。

Different nutrient component in the liquid medium were used to study the effects on the dry weight of fruit body and to get the optimized medium. The results showed that the maxim fruit body yield （14.85 g .L-1） was obtained on the medium of corn starch 1%, wheat bran 1%, glucose 2%, peptone 2%, yeast extract 0.5%, KH2P040.1%, MgS040.05 %, vitamin B1 10 mg-L-1.