摘要
目的探讨TPX2蛋白和mRNA在宫颈鳞癌组织中的表达及其与宫颈鳞癌患者临床病理资料之间的相关性。方法 2009年10月至2010年11月在河北医科大学第四医院,采用免疫组织化学法、逆转录聚合酶链式反应(RT-PCR)法,对62例宫颈癌、15例正常宫颈组织和30例宫颈上皮内瘤变(CIN)组织进行检测,分析TPX2的mRNA和蛋白质的表达。结果经RT-PCR检测,正常宫颈组织中几乎不表达TPX2mRNA,宫颈鳞癌组织中相对含量为0.441±0.011,两者之间差异具有统计学意义(t=-19.568,P<0.01)。经免疫组化检测,TPX2蛋白在正常宫颈鳞状上皮组织中不表达,在CIN和宫颈鳞癌组织中呈阳性表达,三者之间差异具有统计学意义(P<0.05)。宫颈鳞癌组织中TPX2的表达与组织学分级、FIGO分期及淋巴结转移密切相关(P<0.05),而与年龄无关(P>0.05)。结论 TPX2可能参与了宫颈鳞癌的发生和发展,TPX2可作为宫颈鳞癌早期诊断及预后估计的一个指标。
Objective To study the expression of TPX2 protein and mRNA in human cervical squamous carcinoma, and the relationship between its expression and clinicopathologyical features of cervical cancer. Methods Between October 2009 and November 2010 in the Fourth Hospital of Hebei Medical University, Immunohistocbemistry and reverse tran- scription polymerase chain reaction were used to analyze the expression levels of TPX2 mRNA and protein respectively, in fresh invasive cervical squamous cancer (n =62) ,normal cervical uteri (n = 18) and CIN tissues(n =30). Results By RT-PCR, there was no expression of TPX2 mRNA in normal cervical tissues, The expression of TPX2 mRNA cervical carcinoma is 0. 441 ± 0. 011, respectively. There was significant difference among the two tissues (t = -19. 568, P 〈 0.01 ). By immunohistochemistry, normal cervical tissues did not express TPX2. The expression of TPX2 in CIN and cer- vical carcinoma were increased respectively, There was significant difference among the three tissues (P 〈 0. 05 ). The ex- pression of TPX2 were correlated with histologicalgrade, FIGO stages, and lymph nodemetastasis ( P 〈 0. 05 ) and not correlated with age ( P 〉 0. 05 ). Conclusion TPX2 is probably involved in early carcinogenesis of cervical squamous carcinoma. The detection of TPX2 expression may play an important role in early diagnosis and evaluation of prognosis in patients with cervical squamous carcinoma.
出处
《中国实用妇科与产科杂志》
CAS
CSCD
北大核心
2012年第9期685-687,共3页
Chinese Journal of Practical Gynecology and Obstetrics