摘要
以堆腐的发酵牛粪为原料筛选高温纤维素降解细菌.通过纤维素刚果红培养基初筛、测定发酵液的CM-Case和FPAase活力的复筛,筛选出四株酶活较高的菌株,分别是1101、1105、1106和1108.从菌落特征、菌体形态、培养特性及一系列生理生化特性对四株菌株进行初步鉴定,结果表明1101、1106、1108属于芽孢杆菌属,1105属于短芽孢杆菌属.
In order to screen the cellulose-decomposing bacteria, degrading strains were isolated from the cattle ma- nure. Four strains were selected by primary screening of Congo red plate and secondary screening of measurement of CMCase and FPAase activity of fermentation broth. The four strains of 1101, 1105, 1106 and 1108 had efficient degradation capability against the cellulose. Based on the characteristics of colony, morphology, culture and physio-biochemical identification, the strains of 1101,1106,1108 were classified into Bacillus, and 1105 belongs to Brevibacillus Shida.
出处
《河南教育学院学报（自然科学版）》
2012年第3期5-7,10,共4页
Journal of Henan Institute of Education(Natural Science Edition)
基金
河南省科技计划项目(122102310331)
河南省科技计划项目(122102310350)
河南省教育厅自然科学研究计划项目(2011B180015)
河南省教育厅自然科学研究计划项目(2011B180016)
河南省教育厅自然科学研究计划项目(12A180008)
关键词
耐高温的
纤维素降解细菌
筛选
CMCase活力
FPAase活力
鉴定
high-temperature resistant
the cellulose-decomposing bacteria
screening
CMCase activity
FPAaseactivity
identification
