江苏省几种主要类型人畜立克次体抗体阳性检出情况的现况调查

Cross-sectional survey on the prevalence of antibodies to several types of Rickettsia in human and livestock in Jiangsu province

目的了解江苏省人畜立克次体感染的主要类型、抗体阳性检出率及感染的可能影响因素。方法按地理位置,选择江苏省溧水县、沛县、盱眙县、宜兴市和滨海县的5个调查点,采用间接免疫荧光法检测莫氏立克次体(RM)、黑龙江立克次体/西伯利亚立克次体(RH/RS)、恙虫病东方体(OT)、贝氏柯克斯体(CB)、横赛巴尔通体/五日热巴尔通体(BH/BQ)、查菲埃立克体(HME)及人粒细胞无形体(HGA)等7种常见立克次体血清IgG或IgM抗体。结果 5个调查点共取血样2 562份,人群病原体检测率最高的是BH/BQ(24.8%)。男性中HGA和BH/BQ抗体阳性检出率高于女性,其余的检出率是女性高于男性;除了RH/RS外,其余立克次体分布均存在地区差异(均P<0.01)。动物中7种立克次体的抗体均检出,其中OT阳性率最高(31.8%),主要是在羊体内,且存在地区间差异(均P<0.01)。多因素分析发现,影响立克次体抗体阳性率的因素有年龄、性别、地区和蜱/螨叮咬史。结论江苏省农业人群及家畜存在多种立克次体感染,以RM、BH/BQ、CB为多。

Objective To investigate the main types of Rickettsia infection,prevalence of antibodies to Rickettsia and associated factors among human and livestock in Jiangsu province.Methods Five survey sites in different areas in the Province,e.g.Lishui,Peixian,Xuyi,Yixing and Binhai,were selected.Indirect immunofluorescence was applied to detect antibody IgG and IgM to seven common types of Rickettsia such as Rickettsia mooseri（RM）,Rickettsia heilongjiangii（RH）,R.tsutsugamushi,Coxiella burnetii（CB）,Bartonella henselae/Bartonella quintana（BH/BQ）,Ehrlichia chaffeeusis（HME） and Human granulocytic anaplasmosis（HGA）.Results The prevalence of BH/BQ infection was the highest in 2 562 sera in the five survey sites（24.8%）.Prevalence of antibodies was higher in women than that in men,except prevalence of antibodies to HGA and BH/BQ,which was higher in men than that in women.Regional difference was found in all Rickettsia infections except RH/RS.Antibodies to seven types of zoonotic Rickettsia were all detected in animals.The prevalence of OT infection was the highest（31.8%）.It mainly infected goats and the prevalence was different in various areas.Multivariate analysis indicated that age,sex,area and history of tick/mite bite were associated with the prevalence.Conclusions Several types of Rickettsia infection were prevalent in farming population and livestock in Jiangsu province.Infections by RM,BH/BQ and CB were dominant.