X射线照射对小鼠小肠黏膜杯状细胞的影响

Impact of X-Ray irradiation on intestinal mucosa goblet cells in mice

目的通过建立急性辐射损伤动物模型,研究小鼠小肠黏膜急性辐射损伤后的形态学改变及其机制。方法将健康C57BL/6小鼠随机分成正常组和照射组,建立急性辐射损伤模型。照后观察记录小鼠的生存情况,绘制生存曲线;观察小肠黏膜的形态学改变及计数杯状细胞数量;透射电镜观察杯状细胞的超微结构变化;检测小鼠小肠黏膜组织中肿瘤坏死因子(TNF)-α的变化。结果建立急性辐射损伤模型成功;照后小鼠在3d内小肠绒毛被严重破坏并且肠绒毛上杯状细胞显著增多(P<0.01);照后杯状细胞出现了典型的坏死特征;小肠黏膜中TNF-α的含量在照后2 d、3 d后较正常小鼠有显著的下降(P<0.01)。结论小鼠急性辐射损伤后,随着病程的进展,肠道功能受损严重,小肠黏膜中的杯状细胞逐渐增多,同时杯状细胞因照射出现坏死。肠道TNF-α的减少可能加重肠道的辐射损伤效应。

Objective To investigate the morphological change and mechanism of intestinal mucosa after acute radiation damage by the animal model of acute radiation damage.Methods Healthy C57BL/6 mice were randomly divided into normal and radiation group and established the model of acute radiation damage.Draw the kaplan-meier suvival curve of mice after irradiation;observe the pathological change of intestine mucosa and count the number of goblet cells;observe the ultrastructure of goblet cells by transmission electron microscope（TEM）;detect the quantity of tumor necrosis factor（TNF）-α in the intestinal mocusa tissue.Results The model of acute radiation damage was established successfully;the villus of intestinal mucosa were destroyed seriously and the number of goblet cells increased dramaticlly within three days after irradiation（P〈0.01）;the goblet cells have typical features of necrosis after irradiation;the quantity of TNF-α in the intestinal mocusa in 2d and 3d after irradiation is significantly decreased than normal mice（P〈0.01）.Conclusion After acute radiation damage,with the progress of the course of disease,the function of intestine is impaired seriously and the goblet cells in intestine increase gradually.The goblet cells appear necrosis because of irradiation at the same time;the decrease of TNF-α in the intestine aggravates the radiation damage of intestine.