雷公藤内酯醇抑制人前列腺癌DU145细胞增殖和诱导凋亡的体外研究

In vitro study of triptolide inhibit DU145 proliferation and induce apoptosis

目的观察雷公藤内酯醇（TP）对人雄激素非依赖型前列腺癌DUl45细胞增殖、细胞周期及细胞凋亡的影响。方法以不同浓度的雷公藤内酯醇作用于体外培养的DUl45细胞不同的时间，四甲基偶氮唑蓝（MTT）法检测细胞增殖的情况，流式细胞仪检测细胞周期和细胞凋亡的变化。结果20n∥ml、40ng／ml、80ng／mlTP作用DUl45细胞24h、48h、72h后对DUl45细胞均有抑制作用，各组间有显著差异（P〈0．05）；24h、48h、72hIC50分别为117．95ng／ml、47．60ng／ml、14．43ng／ml，TP作用DUl45细胞72min后，DUl45细胞被阻滞于S期，并且随浓度的加大，阻滞效果越显著；凋亡率逐渐增加。结论TP能抑制DUl45细胞的增殖，阻滞DUl45细胞于S期，诱导DUl45细胞调亡。

Objectives To observe the effect of triptolide on prostate cell line DU145 in virto. Methods MTT and FCM were used to observe the effect of different concentration TP on DU145 in different time, detect variation of cell cycle and cell apoptosis. Results 20ng/ml,40ng/ml,80ng,/ml TP inhibited DU145 growth for 24h,48h,72h ;24h,48h,72h IC50 were respectively 117.95 ng/ml,47.60 ng/ml, 14.43 ng/ml, TP block DU145 cell cycle in S stage, induce cell apoptosis. Conclusions TP could inhibit DU145 cell proliferation, block DUI45 cell cycle in S stage, induce cell apoptosis.