昆明山海棠对人精子染色体的诱变作用研究

EFFECTS OF TRIPTERYGIUM HYPOGLAUCUM (LEVEL) HUTCH ON THE CHROMOSOME IN HUMAN SPERMATOZOA IN VITRO

目的与方法 :用人精子染色体畸变试验分析昆明山海棠对人精子染色体畸变率和断裂均数的影响。结果 :结果显示昆明山海棠 (THH) 5 0 μg/ml、10 0 μg/ml、2 0 0 μg/ml3个剂量组的染色体畸变率分别为 11 5 4%、15 71%、16 79% ,断裂均数为 0 .1346、0 .2 143、0 .2 2 14,与空白对照组畸变率 (7.2 5 % )和断裂均数 (0 .10 14)比较 ,除 5 0 μg/ml剂量组外 ,其余 2组均有显著性差异 ,P <0 0 5。结论 :结果表明THH对人精子染色体具有致断作用 。

Purpose and Methods: The effects of Tripterygiu hypoglaucum(Level) hutch(THH) on the induction of chromosome abnormalities in human spermatozoa were studied using the human sperm chromosome assay. Results: Results showed the rates of chromosome aberrations of THH at the doses 50μg/ml, 100μg/ml, 200μg/ml were 11.54%, 15.71%, 16.79% respectively, while 7.25% in control group. There were significant differences 100μg/ml group and 200μg/ml group as compared with control group (P<0.05). The mean value of chromosome breakages of 100μg/ml, 200μg/ml and control groups were 0.2143, 0.2214 and 0.1346 respectively, the difference was significant (P<0.05), when the two tested groups compared with the control group. Conclusion: The study results showed that THH was clastogen in human spermatozoa chromosome aberration test.