不同剂量地西泮对小鼠吗啡镇痛效果的影响

Different Doses of Diazepam on Mice Morphine Analgesia Effect

目的观察不同剂量地西泮对吗啡镇痛效果的影响。方法30只小鼠随机均分为生理盐水组(NS组)、D1组(地西泮8 mg/kg)和D2组(地西泮16 mg/kg),连续注射14 d,第15日停止给药并正常饲喂1 d,第16日各组注射吗啡0.125 mg/kg,分别在地西泮注射及吗啡注射后以热甩尾法测定小鼠痛阈。结果不同剂量地西泮对吗啡镇痛效果的影响有显著性差异,D1组和D2组较NS组痛阈增加(P<0.01),而D1组与D2组间无显著性差异(P>0.05)。结论地西泮能显著增强吗啡的镇痛作用,且可能随剂量的增加增效的幅度有所降低。

Objective To observe the influence of different doses of diazepam on morphine analgesia effect. Methods Thirty mice were randomly divided into 3 groups （n=10）,normal saline （NS） group, D1 group （diazepam 8 mg/kg） and D2 group （diazepam 16 mg/kg）, continuously injected for 14 d, and stopped administration to feed normally on 15 d. Morphine 0. 125 mg/kg was injected in all groups on 16 d. The pain threshold was determined by the heat tail-flick method after injection of diazepam and morphine. Results Different doses of diazepam had significant difference in the influence on the analgesic effect of morphine. The pain threshold of the D1 group was increased as compared with the D2 group, and the pain threshold of the D2 group was higher than that of the NS group. There was no significant difference between D1 and D2 groups. Conclusion Diazepam significantly increases the analgesic effect of morphine, and the increasing amplitude may be decreased to some extent with the dose increase.