门脉高压症患者脾脏及脾静脉壁NOS分布及其意义研究

An investigation of pathological injury of spleen and splenic vein of patients with portal hypertension and its relationship to distribution of NOS

目的 探讨一氧化氮 (NO)在门脉高压症内脏血管病理性损伤中可能的作用。方法 采用光镜 ,电镜及NADPH -黄递酶组织化学染色方法观察门脉高压症患者脾脏及脾静脉病理变化及一氧化氮合酶的分布。结果 与对照组相比门脉高压症患者脾血窦显著扩张 ,脾静脉壁增厚 ,平滑肌增生 ,血管内皮广泛受损 ,脾脏及脾静脉壁内NOS阳性细胞显著增多 ,染色增强。

Aim To investigate the possible role of nitric oxide (NO) in the pathologic injury of splanchnic vascular in portal hypertension.Method The pathologic changes and distribution of NOS in spleen and splenic vein of patients with portal hypertension were studied using light and electron microscpes and NADPH-diaphorase histochemical staining.Results Compared with the control group,the splenic sinus were enlarged notably,the wall of splenic veins were thickened with proliferative smooth muscle cells and the vascular endothelium were impaired massively.And NOS-containing cells were more abundant in distribution and richer in color in spleen and splenic vein of portal hypertension than that of the control group.Conclusions NO may play an important role in the occurrence of portal hypertension.