反应停对急性肝衰竭的的预防作用

The preventive effect of thalidomide on acute liver failure

目的 探讨反应停 (Thal)对急性肝衰竭的预防作用。方法 用D 半乳糖胺 (D GalN ,60 0mg/kg ,ip)和细菌脂多糖 (LPS ,5 μg/只 ,H)复制大鼠急性肝衰竭模型 ,观察Thal对急性肝衰竭死亡率、细胞因子、转氨酶、肝组织形态学和单个核细胞TNFαmRNA表达的影响。结果 Thal可降低急性肝衰竭的死亡率、TNFα和转氨酶的水平 (P <0 .0 5 ) ,减轻肝细胞坏死的程度 ,抑制单个核细胞TNFαmRNA的表达。

Aim To understand the preventive effect of thalidomide on acute live failure.Methods D-Galactosamine (D-GalN,600mg/kg,ip) and lipopolysaccharide(LPS,5μg/rat,H) were used to reproduce the model of acute liver failure on Wistar rats.The mortality of the models was counted.The plasma peak levels of various cytokines and transaminases were measured.The pathological alterations of the livers were examined with hematoxylin and eosin stain under light microscopy.The expression of TNF α mRNA was determined by Dot Blot hybridization combined with semiquantitative analyse.Results It was found that Thal could lower the mortality of the models (P<0.05),reduce the peak levels of plasma cytokines,especially TNF α and transaminases (P<0.05),alleviate the degree of liver injury and supress the expression of TNF α mRNA (P<0.05).Conclusion These results fully demonstrated that Thal had positively preventive effects on acute liver failure as it did in the other diseases.Even though it was found that Thal possessd teratogenic effect during pregnancy,it was still belived that Thal had an important curative significance in the treatment of patients who were not pregnancy.