一种新型肝炎相关性病毒的分子生物学研究

Molecular biological study of a novel DNA virus(TTV) associated with hepatitis in Nanjing area

目的 了解南京地区TTV病毒感染情况 ,重叠TTV感染对慢性乙型肝炎病变程度和HBV复制的影响。方法 采用巢式PCR方法检测血清标本中TTV -DNA。结果 469份血清标本中 ,TTVDNA总检出率为 2 1 1% ( 99/469)。其中健康人群、献血员、血透患者、甲型肝炎、乙型肝炎、丙型肝炎、戊型肝炎、庚型肝炎、非甲 -非庚型肝炎检出率分别是9 4%、3 3 3 %、3 0 8%、11 6%、19 9%、15 4%、8 7%、9 5 %、3 4 1% ;有 2 8例慢性乙型肝炎合并TTV感染 ,轻度、中度和重度慢性乙型患者中TTV检出率无显著差异 ,TTV阳性组和阴性组之间肝功能改变相近。HBeAg、HBVDNA阳性组和HBeAg、HBVDNA阴性组TTVDNA检出率相当。结论 南京地区存在TTV感染 ,TTV是导致非甲 -非庚型肝炎的重要原因 ,TTV可能存在非血源性传播途径 ;

Aim To investigate the infection of transfusion transmitted virus(TTV) in Nanjing, and the influence on the severity of liver disease, hepatitis B virus (HBV) replication in the patients.Methods Nested PCR was applied in the detection of TTV DNA in serum samples.Results Of 469 cases, the total detection rate of TTV DNA was 211%(99/469),of which the detection rates were 9.4% in general individuals, 33.3% in blood donors,30.8% in hemodialysic patients,11.6% in hepatitis A, 19.9% in hepatitis B, 15.4% in hepatitis C, 8.7% in hepatitis E, 9.5% in hepatitis G, 34.1% in non A to G hepatitis, respectively; TTV was found in 28 cases of the patients with chronic hepatitis B, there was no statistical difference in TTV infection among mild、middle and severe chronic hepatitis patients, liver function tests did not differ between TTV positive and TTV negative groups, there was no statistical difference in TTV prevalence between HBeAg/HBV DNA positive and HBeAg/HBV DNA negative groups.Conclusions TTV infection has been found in Nanjing area, TTV may be the key cause of non-A to G hepatitis, it may have non-tranfusion transmitted pathway; TTV infection does not lead to a significant change in severity of chronic hepatitis or HBV replication.