头孢拉定聚合物测定方法的研究

Research on the determination method of cefradine polymer

目的建立了分子排阻色谱方法测定头孢拉定高分子聚合物。方法采用SephadexG-10色谱柱;流动相A:6g.L-1醋酸钠溶液,流动相B:水;流速:1.0mL.min-1;检测波长:254nm;柱温:室温。结果头孢拉定在6.432～75.39mg.mL-1范围内与头孢拉定高分子聚合物峰面积呈良好的线性关系(r=0.998 1)。结论该方法简便、快速、准确可靠,能有效地控制头孢拉定高分子聚合物的含量,可作为头孢拉定高分子聚合物的质量控制方法。

Objective To establish a molecular-exclusion chromatography method for the determination of cefradine polymer. Methods Mobile phase A was 6 g· L^-1 natrium acetum solution,mobile phase B was water,the flow rate was 1.0 mL·min^-1 ,the detection wavelength was 254 nm,and the column temperature was room temperature. Results Calibration curve of cefradine polymer was linear over the range of 6. 432-75.39 mg · mL^-1 （r=0. 998 1）. Conclusion The established method is simple,quick and accurate,it can be used effectively to control the cefradine polymer.