异长春花碱不同制剂的抗肿瘤作用比较研究

Comparison on antitumor effects of different vinorelbine preparations

目的比较异长春花碱(VRB)长循环脂质体、普通脂质体、重酒石酸盐注射液不同剂型的抗肿瘤作用。方法建立C57BL/6J小鼠Lewis肺癌模型,以肿瘤体积、小鼠生存时间、抑瘤率、组织病理切片为指标考察VRB不同制剂的抗肿瘤作用。结果与模型组(平均肿瘤体积为4 980 mm3)相比,VRB长循环脂质体组、普通脂质体组和重酒石酸盐注射液组小鼠造模30 d后的平均肿瘤体积分别为1 622、2 136、3 652 mm3(P<0.05、0.01),抑瘤率分别为66.29%、49.44%、29.21%(P<0.01);VRB长循环脂质体组小鼠生存时间明显延长;肿瘤组织病理切片观察可见各制剂组肿瘤细胞均出现不同程度的坏死,其中VRB长循环脂质体组出现肿瘤细胞核凝固坏死、核破碎溶解现象。结论在相同给药剂量下,VRB长循环脂质体的抗肿瘤作用明显优于其重酒石酸盐注射液。

Objective To compare the antitumor effects ofvinorelbine （VRB） long-circulation liposome （VLCL）, VRB conventional liposome （VCL）, and VRB bitartrate injection （VBI）. Methods Lewis lung carcinoma model （C57BL/6J） was established, and the antitumor effects of different VRB preparations were evaluated with the indexes of tumor volume, survival time, inhibitory rate, and pathological section. Results After 30 d of model establishment, the average tumor volumes of mice in VLCL, VCL ,and VBI groups were 1 622, 2 136, and 3 652 mm3, respectively （P 〈 0.01）. The survival time of mice in VLCL group was obviously prolonged. The tumor inhibitory rate of mice in VLCL, VCL, and VBI groups were 66.29%, 49.44%, and 29.21%, respectively （P 〈 0.05, 0.01）. The tumor cell necrosis in different degrees was found in various administrated groups by using pathological section. Coagulation necrosis, disintegration, and dissolution of tumor cell nucleus could be observed in VLCL group. Conclusion Compared with VBI, VLCL could increase the antitumor effects remarkably in the same doses.