海参磷脂型二十碳五烯酸对链脲佐菌素诱导的糖尿病大鼠肾脏功能改善作用的研究

Protection of EPA-PC liposome on kidney of diabetic rats induced by STZ

目的:研究海参磷脂型二十碳五烯酸(Eicosapentaenoic Acid Phosphatidy lcholine,EPA-PC)对链脲佐菌素(Streptozocin,STZ)诱导的糖尿病大鼠肾脏功能的改善作用。方法:通过腹腔注射STZ建立糖尿病大鼠模型,经不同剂量海参EPA-PC(25和75mg/kg,以EPA含量计)灌胃8w。实验结束后,分别检测大鼠空腹血糖、口服葡萄糖耐量、肾脏指数、24h尿量、血清尿素氮(BUN)、尿总蛋白、白蛋白(mAlb)、尿酸(UA)含量及β-N乙酰葡萄糖苷酶(NAG)活性。结果:海参EPA-PC可显著改善糖尿病大鼠的口服葡萄糖耐量(p<0.05),降低糖尿病大鼠的肾脏指数(p<0.01)、24h尿量、血清BUN、尿总蛋白、mAlb、UA含量及NAG活性(p<0.01)。结论:海参EPA-PC对糖尿病大鼠的肾脏功能具有较好的改善作用。

Objective：To study the protective effects of Eicosapentaenoic Acid Phosphatidylcholine（EPA-PC）on kidney of diabetic rats induced by Streptozocin（STZ）.Methods：Diabetes mellitus induced by STZ were administered by different dosages of EPA-PC（25 mg/kg,75 mg/kg）by stomach perfusion,for 8 weeks.At the end of the experiment,the indices including the changes of the levels of fasting blood glucose,Oral glucose tolerance,the renal index,the 24h urine volume,the levels of BUN in plasma,the excretion of protein,mAlb,BUN,UA and NAG in urine were determined.Results：The EPA-PC showed remarkable hypoglycemic effected on Oral glucose tolerance of diabetic rats（p0.05）,and decreased the renal protein,mAlb,UA and NAG in urine had reduced significantly（p0.01）.Conclusion：EPA-PC can effectively improve the kidney function in diabetic rats.