摘要
[目的]了解济南市手足口病病原学特征,为制定防治措施提供科学依据。[方法]对2011年济南市831例临床诊断手足口病病例标本,采用实时荧光RT-PCR法检测人肠道病毒(PE)、肠道病毒71型(EV71)和柯萨奇病毒A组16型(CoxA16)特异性核酸。[结果]831例手足口病患者标本中,肠道病毒总阳性率为76.05%,其中EV71、CoxA16和其他肠道病毒核酸阳性率分别为40.19%、18.29%和17.57%。肠道病毒阳性率,粪便标本为75.34%,咽拭子为93.75%(P<0.05);3岁最高(83.41%),≥10岁最低(54.55%);男性为77.10%,女性为74.38%(P>0.05);托幼儿童为83.00%,散居儿童为70.60%,学生为52.38%,其他为50.00%;平阴县最高(93.22%),商河县最低(46.88%)。粪便标本阳性率,病后24h内采集的为86.67%,1～3d采集的为83.11%,4～7d采集的为77.52%,8～14d采集的为62.50%,15～21d采集的为51.02%,22～37d采集的为35.71%(P<0.01)。[结论]EV71是济南市2011年手足口病的主要病原体,及时采集标本可以提高阳性检出率。
[Objective]To understand the pathogenic and epidemiological characteristics of hand-foot-mouth disease （HFMD） in Jinan city,so as to provide basis for its control. [Methods]A total of 831 diagnosed HFMD cases were sampled in Jinan city in 2011. The real-time fluorescence quantitative PCR detection of viral nucleic acid was performed with common intestinal virus PE,EV71,and CoxA16. [Results] Among 831 samples, the total positive rate was 76.05%. The positive rate of EV71 ,CoxA16 ,and other HEV was 40.19% ,18.29% and 17.57% respectively. The positive rate of feces and throat swabs was 75.34% and 93.75%（P〈0.05）. The positive rate under three years was the highest（83.41%） ,over ten years was the lowest （ 54.55 % ）. The positive rate of males was 77.10 %, that of females was 74.38 % （ P〉 0.05 ）. The positive rate of kindergarten children, scattered children, students and other populations was 83.00%, 70.60%, 52.38% and 50.00% respectively. The positive rate of Pingyin county was the highest（93.22%） ,that of Shanghe county was the lowest（46.88 %）. The positive rate of feces collected at 24 hours, 1-3 days, 4-7 days, 8-14 days, 15-21 days, and 22-37 days after disease onset was 86.67 %, 83.11%,77.52 %, 62.50 %, 51.02 %, and 35.71% respectively （ P 〈 0.01 ）. [Conclusion]EV71 is the dominant pathogen of HFMD in Jinan city in 2011. Timely collection of samples can increase the positive rate detection.
出处
《预防医学论坛》
2012年第9期646-648,共3页
Preventive Medicine Tribune
