摘要
为了解高油酸甘蓝型油菜突变种质的特点,分析了两组材料营养器官和生殖器官中油酸积累的动态变化。结果表明:营养器官油酸相对含量从高到低排序为根、茎、叶。不同生育期营养器官油酸含量变化表现为:根中,成熟期高,其次是苗期,然后是越冬期、蕾薹期、返青期,花期最低;茎中,成熟期最高,其次是返青期,花期较少,苗期、越冬期和蕾薹期最少;叶中,蕾薹期最高,花期次之,苗期、越冬期和返青期最少。油菜开花2周内,角果中油酸积累以果皮为主,第3周起则转为以种子为主。果皮中油酸相对含量在开花后3周达到最大值。油菜营养器官中的油酸与成熟种子中的油酸相对含量均呈显著正相关(r=0.81*)。除花期外,各生育期营养器官与生殖器官中的油酸相对含量呈极显著正相关(r=0.92**)。表型结果说明供试品系的油酸调控基因是组成型表达,育种上可通过检测营养器官油酸含量来预测和筛选种子高油酸含量的材料。
For high oleic acid(OA) breeding,two series of Brassica napus mutants were used to investigate the dynamic changes of OA accumulation in both vegetative and reproductive organs.Results showed that the relative OA content was high in root,less in stem,and low in leaves.The contents changed in organs at different stages.In root,it was high in mature period,medium in seedling period,and low in flowering period.In stem,it was high in mature period,medium in green-recovering period,and less in flowering period.In leaves,it was high in bolting period,less in flowering period.At the first two weeks after blooming,OA accumulated mainly in silique walls.The relative content in silique walls reached the highest at the 3rd week after flowering,then it accumulated mainly in seeds.The relative OA contents of vegetative organs had significant positive correlation with that of mature seeds(r=0.81).In each period except for flowering,the relative OA contents of vegetative organs had extremely significant positive correlation with that of reproductive organs(r=0.92).The OA accumulation phenotype indicated that genes controlling the OA synthesis in tested mutants were probably constitutive expressed.The positive correlations between vegetative organs and reproductive organs could facilitate early screening of high OA content individuals in breeding program.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2012年第4期359-365,共7页
Chinese Journal of Oil Crop Sciences
基金
国家863计划(2011AA10A104)
江苏省科技支撑计划(BE2009304)
关键词
甘蓝型油菜
高油酸
营养器官
生殖器官
积累
动态变化
Brassica napus L.
High oleic acid
Vegetative organ
Reproductive organs
Accumulation
Dynamics