高肺血流致肺动脉高压幼猪模型中血清内皮素水平的变化

Impact of pulmonary hypertension induced high pulmonary blood flow on endothelin-1 in piglet model

目的研究高肺血流幼猪模型肺动脉血流动力学及组织学变化与血清内皮素的关联性。方法采用生后2-3月龄的幼猪,体重10-12kg,分为两组,每组12只。正常对照组(control group,n=12):即假手术组,只开胸无手术处理;高肺血流组(shunt group,n=12):全部行左侧开胸,降主动脉与左肺动脉搭桥术。所有动物正常饲养3个月。然后正中开胸,检测肺动脉收缩压(PASP)、肺血管阻力(PVR),观察肺动脉标本的结构和组织学变化。Elisa检测血清内皮素水平。结果高肺血流组,肺动脉收缩压(PASP)、肺血管阻力(PVR)显著升高(P<0.01);左肺动脉出现平滑肌细胞显著增生,部分血管中膜及内膜增厚,官腔狭窄;血清内皮素水平升高。结论在幼猪动物模型中,高肺血流可导致肺动脉结构重塑,可导致肺动脉压力的升高,与血清内皮素变化呈正相关。

Objective To research the relation of blood serum endothelin-1（ET1） and pulmonary artery pressure and structure in the high pulmonary blood pig let models.Methods Piglets the age of 2-3 months and weighs 10-12 kg were d ivided into two groups randomly,each group of six.the only Control group（n= 12）,or sham group,only that have not been dealt with the operation of the lung s set;High pulmonary blood group（shunt group,n=12）：all the line left o pen,cut to the left pulmonary artery bypass surgery.All animals are normally rai se three months,then the open,and testing pulmonary arterial systolic pressure （PASP）and pulmonary vascular resistance（PVR）,observing the organization of pulmonary artery;testing ET-1.Results In shunt group,pulmonary arterial syst olic pressure（pasp）and pulmonary vascular resistance（PVR）significantly inc reased（P〈0.01）,Smooth muscle cells in left pulmonary artery were sho wn in remarkably,in part of the tunicae intima vasorum thickend.ET-1 significant ly increased（P〈0.01）;In Control group,no apparent change of pulmonary arterial systolic pressure and pulmonary vascular resistance and ET-1.C onclusion In piglet models,high lung blood flow could induce the resha ping of the p ulmonary artery structure and pulmonary hypertension,could lead to ET-1 signifi cantly increasing.