大肠癌原发灶及粪便标本中基因mRNA表达及其临床意义

Expression of genetic biomarkers mRNA in primary colorectal carcinoma lesion s and exfoliate tumor cells of fecal samples

目的通过对COX-2,CK-20,CD44v6mRNA在结直肠原发灶及病人粪便中的表达,证实粪便中COX-2,CK-20,CD44v6mRNA表达来源于原发灶肿瘤细胞的脱落所致,为临床结直肠癌筛查选择合适的多基因标记物联合检测指标。方法对38例行结肠癌根治术的病人,原发灶及术前收集的粪便标本采用RT-PCR技术对COX-2,CK-20,CD44v6mRNA表达进行检查。结果研究发现,COX-2mRNA在原发灶及粪便中同时阳性表达率为44.7%(17/38),同时阴性表达率为39.5%(15/38),同源性可达84.2%(32/38);CK-20有44.7%(17/38)同时阳性表达,36.8%(14/38)同时无表达,同源性为81.5%(31/38);CD44v6mRNA有50%(19/38)同时阳性表达,44.7%(17/38)同时无表达,同源性为94.7%(36/38),同时性表达差异具有显著性,P值均小于0.001。结论粪便COX-2,CK-20,CD44v6mRNA表达与大肠癌原发灶中的表达具有同源性,说明粪便中COX-2,CK-20,CD44v6mRNA来源于原发灶肿瘤细胞的脱落。

Objective To determine expression of genetic biomarker,RNA-ba sed stool samples originate from the continuously exfoliate tumor cells shed fro m the primary lesions of colorectal cancers or neoplasmas,providing suitable com bined RNA-based stool assays for noninvasive screening test of colorectal neopl asmas or cancers. Methods We carry out the expression of COX-2,CK-20 and CD44v 6mRNA by using RT-PCR in 38 cases of colorectal primary lesions and exfoliate t umor cells of fecal samples. Results We found that the agreed positive expressio n rate of COX-2mRNA was 44.7%（17/38） in two groups samples,the agreed negative expression rate of COX-2mRNA was 39.5%（15/38） in them,their homogenous expressi on rate was 84.2%（32/38）;the agreed positive expression rate of CK-20mRNA was 44.7%（17/38） in the primary colorectal lesions and stool samples,the agreed nega tive expression rate of CK-20 mRNA was 36.8%（14/38）,their homogenous expression rate was 81.5%（31/38）;the agreed positive and negative expression rate of CD44 v6mRNA in the primary lesions and fecal samples were 50%（19/38）,44.7%（17/38）,r espectively,the homogenous expression were statistically significantly differen t from hemogenous expression,P〈0.001,respectively. Conclusion There is a hi gh homogenous expression rate of COX-2,CK-20 and CD44v6mRNA in the primary col orectal cancer lesions and fecal samples,it indicated the expression of genetic biomarkers RNA-based samples originate from the continuously exfoliated tumor cells shed from the primary lesions of colorectal cancer.