庄河大骨鸡ghr、igf-1基因的PCR-RFLP分析及基因聚合与产肉性能的关联分析研究

PCR-RFLP Analysis on ghr,igf-1 Gene and Combination Effects on Meat Performance in Zhuanghe Dagu Chicken

为了探究庄河大骨鸡ghr、igf-1基因聚合与产肉性能相关联的分子标记。利用PCR-RFLP标记技术和最小二乘法分析了ghr、igf-1的多态性及其2基因的聚合基因型与17周龄庄河大骨鸡产肉性能的关联。结果表明:ghr、igf-1基因均存在3种基因型且处在哈代温伯格平衡状态;聚合基因型GGTT、GHTT和GGKT的活重、屠体重、半净膛重、全净膛重、胸肌重、平均日增重显著的高于其他各基因型(P<0.05)。聚合基因型GGTT、GGKT、GHTT对庄河大骨鸡的产肉性能有显著影响,但是否能作为庄河大骨鸡选育的重要理论依据还有待进一步研究。

This study was designed to determine the relation between the meat performance of Zhuanghe Dagu Chicken and combination effects of gbr gene and igK-1 gene. PCR-RFLP technique was used to analysis the polymorphisms of ghr gene and igf-1 gene, while least square method was used to analysis the correlation between the meat performance of the 17 weeks old Zhuanghe Dagu Chicken and specific genotypes as well as the combination effect of ghr, igf-1. It turned out that both of the genes have 3 different genotypes, the frequency of each genotype met the Hardy-weinberg equilibrium. The chickens with the combination genotype GGTr, GHTY and GGKT had significant better performances in terms of live weight, carcass weight, semi-eviscerated weight, eviscerated weight, pectorales weight, average daily gain than those with other genotypes （P〈0.05）. Above all, combination genotype GGTT, GGKT, GHTT of both ghr gene and igf-1 gene contribute significantly to the meat performance of the chickens. Further study was still needed to determine whether these specific genotypes could be taken advantage as candidates in the breeding application.