摘要
为了筛选小尾寒羊母羊FecBBFecBB型与FecB+FecB+型卵巢组织差异表达基因,探讨小尾寒羊品种内两者繁殖力差异的分子生物学机理。本实验利用数字基因表达谱技术分别检测了处于自然发情期的FecBBFecBB型与FecB+FecB+型小尾寒羊卵巢组织的基因表达情况,对两者卵巢组织基因表达谱进行了差异分析,并通过分子注释系统平台DAVID对差异表达基因进行了GO功能显著性富集类分析和Pathway显著性富集分析。结果表明:处于自然发情期FecBBFecBB型比FecB+FecB+型小尾寒羊卵巢组织差异表达基因为238条,主要涉及类固醇生物合成与代谢过程、甾醇生物合成与代谢过程、脂质运输与定位、泛素特异的蛋白酶活性、GTP酶调节活性和Ras蛋白信号转导调节等显著GO功能类和细胞内吞过程、Notch信号通路和嘧啶代谢等显著调控通路。结合已有文献报道,文章初步认为基因STAR、FLCN、TGFI1、INHA、INHBA与Notch信号通路可能是参与调控FecBBFecBB与FecB+FecB+小尾寒羊高低繁殖性状差异的重要基因和调控通路。
Digital Gene Expression Tag Profiling (DGE) technology was used to construct gene expression profile to screen differentially expressed genes of the ovary tissue between FecBSFecBB and FecB+FecB+ ewes of small-tail-han-sheep and investigated the molecular mechanism related to fecundity difference between them. GO (Gene Ontology) and the Pathway analysis were conducted on differentially express genes using a free web-based molecular annotation system DAVID. A total of 238 genes were identified to be differentially express genes. They were mainly involved in steroid biosynthetic and metabolic process, sterol biosynthetic and metabolic process, lipid transport and localization, ubiquitin-specific protease activity, GTPase regulator activity and regulation of Ras protein signal transduction etc. The enriched pathways mainly included endocytosis, Notch signaling pathway and pyrimidine metabolism. According to published papers, the genes including STAR, FLCN, TGFI1, INHA, INHBA and the Notch signaling Pathway were considered as the most important genes and Pathway involved in fecundity differences between FecBSFecB+ and FecB+FecB+ ewes of small-tail-han-sheep.
出处
《中国农学通报》
CSCD
2012年第23期18-25,共8页
Chinese Agricultural Science Bulletin
基金
转基因生物新品种培育科技重大专项"优质高繁转基因肉羊新品种培育"(2009ZX08008-004B
2008ZX08008-003)
关键词
小尾寒羊
FECB基因
卵巢组织
数字基因表达谱
small-tail-ban-sheep
FecB
ovary tissues
digital gene expression tag profiling