农药乐果和杀虫双污染对蚯蚓体内SOD和AChE活性的影响

Effects of Dimethoate and Dimehypo Pollution on Enzymatic Activity of Earthworm

为更好的评价农药对土壤环境的影响,采用蚯蚓毒性试验方法,研究不同浓度乐果和杀虫双对赤子爱胜蚓体内超氧化物歧化酶(SOD)和乙酰胆碱酯酶(AChE)活性的影响。结果表明,蚯蚓受乐果和杀虫双污染后,除一个复合污染外,SOD活性均显著降低(P<0.05),其变化规律与乐果浓度和复合污染浓度之间关系不明显,而随杀虫双浓度的升高,总体呈现出降低的趋势,处理时间(24h和48h)之间存在显著差异(P<0.05);AChE活性受乐果污染后,呈现出极显著(P<0.01)的抑制作用,而受杀虫双污染的影响无明显规律,复合污染均显著(24h)或极显著(48h)抑制AChE活性。因此,可以用蚯蚓体内SOD来间接评价杀虫双对土壤的污染,而AChE活性能更好的评价乐果对土壤的污染。

For better evaluating the effects of pesticide on soil Environment, the method of earthworm toxicity test was used to study the earthworm＇ s activity of superoxide dismutase （SOD） and acetylcholinesterase （ACHE） impacted by different concentration pollution of dimethoate and dimehypo. The results showed that the SOD activity of earthworm was significantly lower （P〈O.05） than that of CK after contaminated by dimethoate and dimehypo expect for one of combined procession, and it had no significant relationship between the variation of SOD activity and the concentration of dimethoate and the concentration of compound contamination, but showed a decreasing trend with the increase of dimehypo＇ s concentration overall, there were significant differences （P〈0.05） between contamination time （24 h and 48 h）. The AChE activity of earthworm showed a very significant （P〈0.01） inhibition contaminated by dimethoate, and it didn＇t show obvious regularity by dimehypo, the AChE activity of earthworm was significantly （24 h） or very significantly （48 h） inhibited by compound contamination. Therefore, the earthworms SOD could be used to evaluate the soil contamination by dimehypo indirectly, and earthworm＇ s AChE activity could be a better evaluation quota on soil pollution by dimethoate.