内皮型氧化亚氮合酶基因G894T、胰岛素受体基因第17外显子多态性与老年痴呆的关系研究

Relationship between polymorphism of insulin receptor gene exon 17,endothelial nitric oxide synthase gene G894T and Alzheimer′s disease

目的研究内皮型氧化亚氮合酶基因第7外显子G894T、胰岛素受体基因第17外显子多态性与老年痴呆的相关性。方法选取本院门诊及住院阿尔茨海默病(AD组)与血管性痴呆患者(VaD组)各50例。对照组为同期收集的健康老年人50例,抽取受试者清晨空腹静脉血5 mL,血细胞提取DNA。对受试者的内皮型氧化亚氮合酶基因G894T和胰岛素受体基因第17外显子进行基因分析。结果 AD组、VaD组与对照组的GG、GT和TT基因型分布频率分别比较,差异有显著统计学意义;AD组患者的INSR T等位基因频率高于对照组,但差异不明显(P>0.05)。VAD组患者的INSR T等位基因频率显著高于对照组。结论内皮型氧化亚氮合酶基因G894T多态性、胰岛素受体基因第17外显子多态性与阿尔茨海默病、血管性痴呆患者具有相关性,AD和VaD存在相同的基因危险因素。

Objective To study the relationship between polymorphism of insulin receptor gene exon 17,endothelial nitric oxide synthase gene G894T and Alzheimer’s disease.Methods A total of 100 patients with Alzheimer’s disease（AD） and vascular dementia（VaD） in our hospital were selected and divided into AD group（n=50） and VaD group（n=50）.Another 50 healthy subjects were selected as control group.They were subjected to drawing blood of 5 mL with an empty stomach in the morning,and the DNA was extracted from blood cells.The polymorphism of insulin receptor gene exon 17 and endothelial nitric oxide synthase gene G894T were analyzed.Results There were statistically significant differences in the GG,GT and TT genotype distribution frequency between the AD group,the VaD group and the control group.The INSR T-allele frequency in the AD group was higher than that in the control group,but the difference was not significant（P〉0.05）.The INSR T-allele frequency in the VAD group was significantly higher than that in the control group.Conclusion The polymorphism of insulin receptor gene exon 17 and endothelial nitric oxide synthase gene G894T are correlated with Alzheimer′s disease and vascular dementia.AD and VaD have the same genetic risk factors.