犏牛主要组织相容性复合物DRA基因(MHC-ClassⅡ-DRA)的分子特征分析

Analysis of the Molecular Characteristics of Chinese Yakow Major Histocompatibility Complex DRA Gene(MHC-ClassⅡ-DRA)

为探索犏牛的抗病特性,本试验运用1对引物首次扩增出了犏牛DRA基因的编码区全长,GenBank登录号为:JQ347519。通过NCBI开放阅读框寻找功能模块等生物信息学软件分析后结果表明,犏牛DRA基因编码区全长762bp,编码253个氨基酸。对犏牛DRA基因编码产物进行了疏水性、信号肽、N-糖原区和二级结构预测,分析了犏牛DRA基因与其他物种的同源性,与黄牛相比,犏牛DRA基因表现出高度同源,同源性达到了99.0%。与MHC家族其他基因不同,DRA基因在所有的动物尤其是反刍动物中表现出高度保守,包括肽结合部位(PBS)、N-糖原区、α1、α2区等功能部位都呈现出高度保守。

To explore the resistance traits of Chinese Yakow,in the present study,the Yakow coding region sequence of the DRA gene had been amplified by one pair primer and deposited into the GenBank database under accession JQ347519.According to the ORF FINDER from NCBI and other bioinformatics tools,the length of the coding region was 762 bp which encoded 253 amino acids.Moreover,the characteristics of hydrophobicity,signal peptide,N-linked glycosylation and the secondary structure were predicted.Analysis the homology with other species and compared with cattle,Yakow DRA（bogr×bola-DRA） sequence showed highest similarity（99.0%）.Unlike other MHC molecules,the DRA gene showed highly conserved characteristics across all animals especially ruminants in functional positions,such as antigen binding at peptide binding site（PBS）,N-linked glycosylation,α1 and α2 domains.