口蹄疫病毒完整病毒粒子定量方法的研究进展

Development of Quantification Methods of Foot and Mouth Disease Virus Whole Virus Particles

口蹄疫疫苗的有效抗原成分为完整的口蹄疫病毒粒子(146S),疫苗的效力与疫苗中含有的146S具有极大的相关性。因此对疫苗的生产过程及产品进行146S的定量检测是非常重要的环节。目前对146S定量的方法主要是超速离心的方法,除此之外,ELISA检测也越来越受到关注,同时很多研究人员也在积极探索其他更为简便快捷的检测方法。作者主要对各种146S的定量方法作一综述。

The effective component of FMD vaccine is the whole virus particles of FMDV.The potency of vaccine is correlated to the amount of the 146S in the vaccine.It is very important to detection and quantification 146S in the vaccine and during the process of vaccine producing.Currently,the main method is ultra speed centrifugation.In addition,ELISA is concerned more and more.In the same time,many researchers are exploring other methods that may be simpler and faster.In this paper,various methods are introduced.