猪繁殖与呼吸综合征、猪圆环病毒2型、猪伪狂犬病病毒混合感染的检测分析

Detection and Analysis of Porcine Circovirus,Pseudorabies Virus Mixed Infection with Porcine Reproductive and Respiratory Syndrome Virus

本研究采用RT-PCR方法对2010年12月至2011年11月采集于豫西地区的170份疑似PRRSV感染猪病料进行病原检测,得到102份高致病性PRRSV阳性样本,在此基础上应用PCR方法检测PCV2和PRV的感染情况,并计算混合感染率。试验结果显示,豫西地区PRRS发病猪主要疫病的总混合感染率为58.52%,二重混合感染率为39.21%,三重混合感染率为19.61%,其中PRRSV/PCV2型二重混合感染最严重,混合感染率达33.33%;春夏和秋冬总混合感染率分别为48.57%、81.25%,而且不管是二重还是三重混合感染,秋冬均比春夏更严重,尤其是PRRSV/PCV2/PRV型三重混合感染,秋冬与春夏季节的混合感染率相差较大,分别为37.50%、11.43%;PRRS发病猪从哺乳期到育肥期都有混合感染,混合感染率分别为42.10%、50.00%、100.00%,混合感染程度逐渐加重,主要集中在育肥期;不同发病时期的最高混合感染型也有所不同,其中哺乳期最高混合感染型为PRRSV/PCV2,混合感染率为42.10%,保育期最高混合感染型为PRRSV/PCV2、PRRSV/PCV2/PRV,混合感染率均为22.73%,育肥期最高混合感染型为PRRSV/PCV2/PRV,混合感染率为50.00%。本研究反映了豫西地区PRRS发病猪群与猪圆环2型及猪伪狂犬病的混合感染情况和规律,为该地区PRRS及其混合感染的临床诊治和区域性防控工作的进行提供了理论依据和指导。

The 170 clinic suspected PRRS samples collected in the western region of Henan province from December 2010 to November 2011 were tested by RT-PCR.The PCR was used to detect PCV2 and PRV from the 102 HP-PRRS positive samples.The detection results showed that the total mixed infection rate was 58.52%,the double mixed infection rate was 39.21%,and the triple mixed infection rate was 19.61%.The PRRSV/PCV mixed infection rate was the highest,which was 33.33%.In spring and summer,autumn and winter season,the mixed infection rates were 48.57% and 81.25% respectively.The mixed infection was more serious in the autumn and winter than in the spring and summer whether the double or the triple mixed infection.Especially the triple mixed infection rates about PRRSV/PCV2/PRV were 11.43% and 37.50% in spring and summer,autumn and winter.There were mixed infection from suckling to fattening period,the mixed infection rates of PRRS were 42.10%,50.00% and 100.00%.There was different mixed infection at different stages of swine.The chief mixed infection at suckling period was PRRSV/PCV2（42.10%）,the chief mixed infection at nursery period was PRRSV/PCV2 or PRRSV/PCV2/PRV（22.73%,22.73%）,and the chief mixed infection at fattening period was PRRSV/PCV2/PRV（50.00%）.This research reflected the situation and the rule of the mixed infection（PRRSV,PCV2 and PRV）,and provided theoretical basis and guidance for clinical diagnosis and treatment of mixed infection and regional prevention in western region of Henan province.