上海某社区居民叶黄素膳食摄入量与血清含量的相关性分析

Correlation Analysis on Dietary Lutein Intake and Serum Lutein Concentration of Residents in a Community of Shanghai

[目的]了解某社区40岁～居民叶黄素营养水平,分析膳食叶黄素摄入量和血清叶黄素含量之间有无相关性。[方法]将341名调查对象分为40岁～、50岁～和60岁～年龄组。采用24h和食物定量频率法问卷法进行膳食调查,计算出平均每人每日膳食叶黄素摄入量。采用高效液相色谱法检测血清叶黄素含量。并对膳食叶黄素摄入量和血清叶黄素含量进行相关性分析。[结果]血清叶黄素在0.08～1.6μg/mL范围内线性良好,相关系数为r=0.9973,回收率为97.85%～103.67%,日内和日间变异系数分别为2.32%和3.08%,最低检测限为0.025μg/mL。蔬菜是调查对象膳食叶黄素的主要来源,尤其是菠菜、韭菜、西兰花;膳食叶黄素摄入量和血清叶黄素含量分别为(7.77±2.10)mg/d和(0.370±0.183)μmol/L,且60岁～组均明显低于50岁～组(P<0.05);膳食叶黄素摄入量与血清三酰甘油呈负相关(r=-0.108,P<0.05),与血清胆固醇呈正相关(r=0.231,P<0.001),与血清叶黄素含量呈正相关(r=0.681,P<0.001)。[结论]膳食和血清叶黄素明显相关,后者可以作为反映叶黄素营养状况的指标。老年人更应注重叶黄素的摄入和补充。

[ Objective ] To assess lutein levels of residents aged 40 years and older, and to determine whether dietary lutein intake is correlated with serum lutein concentration. [ Methods ] Three hundred and forty-one, enrolled participants were divided into three groups based on age, namely, 40-49, 50-59, and 〉60 years groups, and completed a quantitative food frequency questionnaire with 24-hour dietary recalls. Data from the questionnaires were used to calculate daily lutein intake. Serum concentrations of lutein were determined by high performance liquid chromatography. Then the correlation between dietary lutein intake and serum lutein concentration was evaluated. [ Results ] The linearity of lutein was good within the range of 0.08-1.6μg/mL, the correlation coefficient was r=0.997 3, the recovery rate was 97.85%-103.67%, intra-day and inter-day coefficients of variation were 2.32% and 3.08% respectively, and the detect limit was 0.025μg/mL. Vegetables were the leading sources of lutein in the participants, especially spinach, leek, and broccoli. The average dietary lutein intake was （7.77 ±2.10） mg/d, the average serum lutein concentration was （0.370 ± 0.183）μmol/L, and both were significantly lower in the 〉60 years group than in the 50-59 years group （P 〈 0.05）. The dietary lutein intake was negatively correlated with the triglyceride concentration （r=--O. 108, P 〈 0.05）, and positively correlated with the cholesterol concentration （r=0.231, P〈0.001） and the serum lutein concentration （r=0.681, P〈0.001）. [ Conclusion ] There is a positive correlation between dietary lutein intake and serum lutein concentration, and the latter could be used as an indicator of individual lutein level. Elderly people should consider improving intake or supplement of lutein.