白藜芦醇对力竭游泳大鼠肝脏组织的保护作用研究

Research on the Resveratrol's Proctection Function on the Rats' Liver After the Exhaustive Swimming

目的:探讨白藜芦醇对肝脏组织的保护作用,进而为白藜芦醇应用于运动领域提供实验依据。方法:选取雄性SD大鼠30只,随机分为3组,每组10只,分别为安静对照组、运动对照组和实验组(运动+100mg/kgRes)。各组大鼠每日称重后,实验组每天灌胃100mg/kgRes,其他两组分别灌胃相同体积的溶酶,连续2周。于第15天,运动对照组和实验组进行力竭游泳,运动后即刻,测定血清中谷丙转氨酶(ALT)活性和肝脏组织中超氧化物歧化酶(SOD)活性、总抗氧化能力(T-AOC)和丙二醛(MDA)含量。结果:运动对照组大鼠肝脏组织MDA含量显著高于安静对照组(p<0.01),实验组肝脏组织MDA含量显著低于运动对照组(p<0.01);运动对照组大鼠肝脏组织SOD活性显著低于安静对照组(p<0.01),实验组肝脏组织SOD活性显著高于运动对照组(p<0.01);运动对照组大鼠肝脏组织T-AOC水平显著高于安静对照组(p<0.05),实验组肝脏组织T-AOC水平显著高于运动对照组(p<0.01);运动对照组大鼠血清中ALT水平显著高于安静对照组(p<0.01),实验组血清中ALT水平显著低于运动对照组(p<0.01)。结论:①力竭运动后,机体内源性自由基水平迅速升高,造成肝脏组织损伤。②补充白藜芦醇能够增强肝脏组织抗氧化能力,起到保护肝脏的作用。

Purpose： This paper discussed the protective function of resveratrol on liver tissue, and thus provided the experimental basis for resveratrol used in the sports field. Method： The study selected 30 male SD rats and randomly divided them into 3 groups. Each group had 10 rats and they were quiet control group, exercise control group and experimental group （motion ＋100 mg/kgRes）. After daily weighing of the rats, the experimental group was intragastrie administration 100 mg/kgRes one time each day, and the two other groups were filled the same volume of enzyme respectively for 2 consecutive weeks. On the 15th day, we conducted exhaustive swimming in the exercise control group and experimental group, and instantly measured ALT ac tivity in serum and SOD activity, T-AOC and MDA content in the liver tissue. Results： The MDA content of the rats＇ liver tissue in the exercise control group is significantly higher than the quiet control group （p〈0.01）, while the MDA content of the experimental group is significantly lower than the control group （p〈0. 01）. The SOD activity of the rats＇ liver tissue in the con- trol group is significantly lower than the quiet control group （p〈0.01）, while in the experimental group the SOD activity of the liver tissue is substantially higher than the exercise control group （p〈0. 01）. In the exercise control group the TAOC level of the rats＇ liver tissue is significantly higher than the quiet control group （p〈0. 05）, while in the experimental goup the T-AOC level of the liver is substantially higher than the exercise control group （p〈0.01）. In the exercise control group the ALT level in the rats＇ serum is substantially higher than the quiet control group （p〈0. 01）, while in the experimental group the ALT level is significantly lower than the exercise control group （p〈0.01）. Conclusion： After the exhaustive exercise, the endogenous free radical level of the body rises rapidly, causing liver injury. The supplement of resveratrol can enhance liver tissue antioxidant cw pacity and protect the liver.