聚己内酯-吐温80共聚物纳米粒在神经胶质瘤化疗中的应用

Docetaxel-loaded PCL-Tween 80 nanoparticles for glioma chemotherapy

目的研究新型载多西紫杉醇聚己内酯一吐温80共聚物（PCL—Tween80）纳米粒在神经胶质瘤化疗中的应用。方法以PCL—Tween80和聚己内酯为材料，利用改良的溶剂萃取／挥发方法制备载多西紫杉醇纳米粒并进行性质表征。利用激光共聚焦显微镜观察纳米粒的细胞摄取情况，并利用噻唑蓝（MTT）法测定纳米粒对c6细胞的细胞毒作用。结果载药纳米粒呈球形，粒径约为200nm。PCL-Tween80纳米粒的载药量为10％，28d内可以释放包裹药物的34．90％。与同浓度的泰素帝（Taxotere~）比较，载多西紫杉醇PCL—Tween80纳米粒对C6细胞的细胞毒性作用更强。结论载多西紫杉醇PCL．Tween80纳米粒用于神经胶质瘤的化疗极具应用前景。

Objective To investigate the feasibility of using a novel docetaxel-loaded polycaprolactone- Tween 80 （PCL-Tween 80） nanoparticles for glioma therapy. Methods Two types ofdocetaxel-loaded nanoparticles were made from commercial polycaprolactone （PCL） and a self-synthesized PCL-Tween 80 copolymer using a modified solvent extraction/evaporation method. A C6 gli0ma cell line was used to investigate the uptake of polymeric nanoparticles by brain cancer cells. In vitro cancer cell viability was assessed using MTF toxic assay. Results The nanoparticles were found by FESEM to have a spherical shape and be around 200 nm in diameter. The copolymers could encapsulate 10% of the drug in the nanoparticles and release 34.9% of the encapsulated drug over 28 days. The drug-loaded PCL-Tween 80 nanoparticles showed better in vitro cytotoxicity towards C6 cancer cells than Taxotere at the same drug concentration. Conclusion Nanoparticles using PCL-Tween 80 copolymer as drug delivery vehicles may have a promising outcome for glioma patients.